Cytoplasmic Polyhedrosis Virus Classification by Electropherotype; Validation by Serological Analyses and Agarose Gel Electrophoresis
Mertens, Peter P. C. and Crook, Norman E. and Rubinstein, Riva and Pedley, Steven and Payne, Christopher C.,, 70, 173-185 (1989),
doi = https://doi.org/10.1099/0022-1317-70-1-173,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= SUMMARY Serological analyses of several different cytoplasmic polyhedrosis viruses (CPVs), including two type 1 CPVs from Bombyx mori, type 1 CPV from Dendrolimus spectabilis, type 12 CPV from Autographa gamma, type 2 CPV from Inachis io, type 5 CPV from Orgyia pseudotsugata and type 5 CPV from Heliothis armigera, demonstrated a close correlation between the antigenic properties of the polyhedrin or virus particle structural proteins and the genomic dsRNA electropherotypes. The dsRNAs of these viruses were analysed by electrophoresis in 3% and 10% polyacrylamide gels with a discontinuous Tris–HC1/Tris–glycine buffer system or by 1% agarose gel electrophoresis using a continuous Tris–acetate–EDTA buffer system. Electrophoretic analysis in agarose gels was found to be the most suitable for the classification of CPV isolates into electropherotypes, and the results obtained showed a close correlation with the observed antigenic relationships between different virus isolates. However, electrophoretic analysis in 10% polyacrylamide gels was most sensitive for the detection of intra-type variation and the presence of mixed virus isolates.,
language=,
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