1887

Abstract

SUMMARY

A small RNA (DI RNA), approx. 400 bp in length, was found in plants inoculated with cymbidium ringspot tombusvirus (CyRSV). DI RNA contained sequences derived from at least three regions of the CyRSV genome and was encapsidated in CyRSV protein. DI RNA was formed in plants inoculated with purified genome-size RNA and a double-stranded form was detected in dsRNA extracted from infected tissue. Symptoms of CyRSV infection in were less severe when DI RNA was present in the inocula used suggesting that DI RNA interferes with symptom expression.

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1989-01-01
2024-04-23
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References

  1. Barrett A. D. T., Dimmock N. J. 1986; Defective interfering viruses and infections of animals. Current Topics in Microbiology and Immunology 128:55–84
    [Google Scholar]
  2. Chen R. J., Seeburg P. H. 1985; Supercoil sequencing: a fast simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  3. Diaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral double-stranded RNA using a LiCl fractionation procedure. Preparative Biochemistry 8:1–7
    [Google Scholar]
  4. Gallitelli D., Hull R. 1985; Characterization of satellite RNAs associated with tomato bushy stunt virus and five other definitive tombusviruses. Journal of General Virology 66:1533–1543
    [Google Scholar]
  5. Hillman B. I., Schlegel D. E., Morris T. J. 1985; Effect of low molecular weight RNA and temperature on tomato bushy stunt virus symptom expression. Phytopathology 75:361–365
    [Google Scholar]
  6. Hillman B. L., Carrington J. C., Morris T. J. 1987; A defective interfering RNA that contains a mosaic of ‘ plant virus genome. Cell 51:427–433
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Rigby P. W. I., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  9. Russo M., Burgyan J., Carrington J. C., Hillman B. I., Morris T. J. 1988; Complementary DNA cloning and , characterization of cymbidium ringspot virus RNA. Journal of General Virology 69:401–406
    [Google Scholar]
  10. Taylor J. M., Illmensee R., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:325–330
    [Google Scholar]
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