1887

Abstract

SUMMARY

Foot-and-mouth disease virus (FMDV) A Iraq 24/64 adapted to grow in BHK monolayer cells induced antibodies which neutralized many isolates belonging to the A serotype. Plaque-purified virus isolated from this stock also induced broadly reactive antibodies, showing that this property is not due to the combined response to a mixture of variants in the original stock virus. However, viruses obtained by passage in suspension BHK cells of either the monolayer cell-adapted virus or a virus cloned from this stock resulted in the selection of virus which induced antibodies with highly specific neutralizing activity. In addition to their antigenic properties the monolayer and suspension cell-adapted viruses could be distinguished by plaque morphology, tendency to aggregate and ability to attach to BHK cells. Monoclonal antibodies (MAbs) induced with the plaque-purified monolayer-adapted virus had neutralizing activity almost as broad as polyclonal serum, showing that this property can be represented by a single epitope on the virus. These neutralizing MAbs recognize a trypsin-sensitive epitope on the virus. Surprisingly, sequence analysis of the structural protein-coding regions of the genomic RNAs of monolayer and suspension cell-adapted viruses showed no amino acid differences in VP1, the protein known to contain the major neutralization epitope in FMDV and to be the only protein susceptible to cleavage by trypsin in the virus particle. Although three coding differences were found in the capsid protein these were all located in VP2.

Keyword(s): capsid protein , cell selection and FMDV
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1989-01-01
2024-03-29
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References

  1. Acornley J. E., Chapple P. J., Stott E. J., Tyrrell D. A. J. 1968; Selection of variants of rhinoviruses. Archiv für die gesamte Virusforschung 23:284–287
    [Google Scholar]
  2. Arrowsmith A. E. M. 1975; Variation among strains of type A foot-and-mouth disease virus in the Eastern Mediterranean region 1964-1972. Journal of Hygiene 75:387–397
    [Google Scholar]
  3. Bahnemann H. G. 1973; The inactivation of FMDV by ethylenimine and propylenimine. Zentralblatt für Veterinärmedizin 20:356–360
    [Google Scholar]
  4. Baxt B., Morgan D. O., Robertson B. H., Timpone C. A. 1984; Epitopes on foot-and-mouth disease virus outer capsid protein VP1 involved in neutralization and cell attachment. Journal of Virology 51:298–305
    [Google Scholar]
  5. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature London: 29830–33
    [Google Scholar]
  6. Bolwell C., Clarke B. E., Parry N. R., Ouldridge E. J., Brown F., Rowlands D. J. 1989; Epitope mapping of foot-and-mouth disease virus with neutralizing monoclonal antibodies. Journal of General Virology 70:59–68
    [Google Scholar]
  7. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature London: 1991168–1170
    [Google Scholar]
  8. Carroll A. R., Rowlands D. I., Clarke B. E. 1984; The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus. Nucleic Acids Research 12:2461–2472
    [Google Scholar]
  9. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. 1982; The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (HI subtype). Cell 31:417–427
    [Google Scholar]
  10. Clarke B. E., Sangar D. V., Burroughs J. N., Newton S. E., Carroll A. R., Rowlands D. J. 1985; Two initiation sites for foot-and-mouth disease virus polyprotein in vivo. Journal of General Virology 66:2615–2626
    [Google Scholar]
  11. Cowan K. M., Erol N., Whitehead A. P. 1974; Heterogeneity of type Asia 1 foot and mouth disease virus and BHK-21 cells and the relationship to vaccine preparation. Bulletin.Office international des épizooties 81:1271–1289
    [Google Scholar]
  12. Hare J. D., Morgan H. R. I. 1962; A polyoma virus variant with new antigenic determinants. Virology 19:105–107
    [Google Scholar]
  13. Mccullough K. C., Crowther J. R., Butcher R. N. 1985; Alteration in antibody reactivity with foot-and-mouth disease virus (FMDV) 146S antigen before and after binding to a solid phase or complexing with specific antibody. Journal of Immunological Methods 82:91–100
    [Google Scholar]
  14. Mcmaster W. R., Williams A. F. 1979; Identification of glycoproteins in rat thymus and purification from rat spleen. European Journal of Immunology 9:426–433
    [Google Scholar]
  15. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual. New York:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Martinsen J. S. 1971; Two plaque-size variants of foot-and-mouth disease virus differing in neutralization by guinea-pig antisera. Research in Veterinary Science 12:399–400
    [Google Scholar]
  18. Messing J., Vieira J. 1982; A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19:269–276
    [Google Scholar]
  19. Morrell D. J., Mellor E. I. C., Rowlands D. L., Brown F. 1987; Surface structure and RNA-protein interactions of foot-and-mouth disease virus. Journal of General Virology 68:1649–1658
    [Google Scholar]
  20. Onishchenko A. M., Petrova N. A., Blinov V. M., Vassilenko S. K., Sandakhchiev L. S., Burdov A. N., Ivanyushchenkov V. N., Perevozchikova N. A. 1986; The DNA-copy primary structure for the gene of the FMDV A22 serotype VP1-protein. Bioorganicheskdya Khimiya 12:416–419
    [Google Scholar]
  21. Ouldridge E. J., Barnett P. V., Rweyemamu M. M. 1982; The relative efficiency of two ELISA techniques for the titration of FMDV antigen. Veterinary Medicine and Animal Science 22:142–151
    [Google Scholar]
  22. Ouldridge E. J., Barnett P. V., Parry N. R., Syred A., Head M., Rweyemamu M. M. 1984; Demonstration of neutralizing and non-neutralizing epitopes on the trypsin-sensitive site of foot-and-mouth disease virus. Journal of General Virology 65:203–207
    [Google Scholar]
  23. Parry N. R., Rweyemamu M. M., Pay T. W. F. 1978; Studies of plaque variants of foot-and-mouth disease vaccine virus. Bulletin. Office international des épizooties 89:929–943
    [Google Scholar]
  24. Parry N. R., Ouldridge E. J., Barnett P. V., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1985; Identification of neutralizing epitopes of foot-and-mouth disease virus. In Vaccines ’85211–216 Lerner R. A., Chanock R. M., Brown F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Pfaff E., Mussgay M., Bohm H. O., Schulz G. E., Schaller H. 1982; Antibodies against a preselected peptide recognize and neutralize foot and mouth disease virus. EMBO Journal 7:869–874
    [Google Scholar]
  26. Robertson B. H., Morgan D. O., Moore D. M. 1984; Location of neutralizing epitopes defined by monoclonal antibodies generated against the outer capsid polypeptide, VP1, of foot-and-mouth disease virus A12. Virus Research 1:489–500
    [Google Scholar]
  27. Robertson B. H., Grubman M. J., Weddell G. N., Moore D. M., Welsh J. D., Fischer T., Dowbenko D. J., Yansura D. G., Small B., Kleid D. G. 1985; Nucleotide and amino acid sequence coding for the polypeptides of foot and mouth disease virus type A12. Journal of Virology 54:651–660
    [Google Scholar]
  28. Rowlands D. J., Clarke B. E., Carroll A. R., Brown F., Nicholson B. H., Bittle J. L., Houghten R. A., Lerner R. A. 1983; Chemical basis of antigenic variation in foot-and-mouth disease virus. Nature London: 306694–697
    [Google Scholar]
  29. Rweyemamu M. M. 1984; Antigenic variation in foot-and-mouth disease: studies based on the virus neutralization reaction. Journal of Biological Standardization 12:323–337
    [Google Scholar]
  30. Rweyemamu M. M., Booth J. C., Head M., Pay T. W. F. 1978; Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains. Journal of Hygiene 81:107–123
    [Google Scholar]
  31. Rweyemamu M. M., Ouldridge E. J., Head M., Purse F. 1984; Evaluation of the antigenic variation within type A foot-and-mouth disease virus isolates from Asia. Journal of Biological Standardization 12:191–194
    [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 74:5463–5467
    [Google Scholar]
  33. Schild G. C., Oxford J. S., De iong J. C., Webster R. G. 1983; Evidence for host-cell selection of influenza virus antigenic variants. Nature London: 303706–709
    [Google Scholar]
  34. Sobrino F., Palma E. L., Beck E., Davila M., De La Torre J. C., Negro P., Villanueva N., Ortin J., Domingo E. 1986; Fixation of mutations in the viral genome during an outbreak of foot-and-mouth disease: heterogeneity and rate variations. Gene 50:149–159
    [Google Scholar]
  35. Stave J. W., Card J. L., Morgan D. O. 1986; Analysis of foot-and-mouth disease virus type O1 Brugge neutralization epitopes using monoclonal antibodies. Journal of General Virology 67:2083–2092
    [Google Scholar]
  36. Strohmaier K., Franze R., Adam K.-H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  37. Totsuka A., Ohtaki K., Tagaya I. 1978; Aggregation of enterovirus small plaque variants and polioviruses under low ionic strength conditions. Journal of General Virology 38:519–533
    [Google Scholar]
  38. Weddell G. N., Yansura D. G., Dowbenko D. J., Hoatlin M. E., Grubman M. J., Moore D. M., Kleid D. G. 1985; Sequence variation in the gene for the immunogenic capsid protein VP1 of foot and mouth disease virus type A. Proceedings of the National Academy of SciencesU.S.A. 82:2618–2622
    [Google Scholar]
  39. Wild T. F., Burroughs J. N., Brown F. 1969; Surface structure of foot-and-mouth disease virus. Journal of General Virology 4:313–320
    [Google Scholar]
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