f Recombinant Vaccinia Virus Expressing the Herpes Simplex Virus Type 1 Glycoprotein C Protects Mice against Herpes Simplex Virus Challenge
- Authors: Jerry P. Weir, Malcolm Bennett†, Elizabeth M. Allen, Karen L. Elkins, Stephen Martin, Barry T. Rouse
- First Published Online: 01 October 1989, Journal of General Virology 70: 2587-2594, doi: 10.1099/0022-1317-70-10-2587
- Subject: Animal
- Issue Published:
The gene encoding the herpes simplex virus type 1 (HSV-1) glycoprotein C (gC) was isolated and cloned into a vaccinia virus insertion vector, and the resulting vaccinia-gC vector was used to construct a recombinant vaccinia virus that expressed gC (VVgC5). Infection of cells with VVgC5 resulted in cell surface expression of authentic HSV-1 gC. HSV-1 gC-specific neutralizing antibodies were produced in VVgC5-immunized mice, and lymphocytes exhibited an HSV-1-specific proliferation response in vitro following infection. More importantly, VVgC5-immunized mice were resistant to subsequent lethal HSV-1 challenge.
Present address: Department of Veterinary Pathology, University of Liverpool, Neston, Merseyside L64 7TE, U.K.
© Society for General Microbiology 1989 | Published by the Microbiology Society
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