1887

Journal of General Virology header logo

Volume 70, Issue 12

Competition between Isolates and Variants of Cauliflower Mosaic Virus in Infected Turnip Plants Free

Abstract

SUMMARY

The Cabb S isolate of cauliflower mosaic virus (CaMV) is the only isolate that can be recovered from turnip plants mixedly infected with Cabb S and an infectious variant of Cabb S. Analysis of the progeny resulting from mixed infections of turnip plants was done for a better understanding of this dominance. Cabb S DNA was the dominant DNA recovered from plants after a mixed infection with Cabb S and D/H or W isolates of CaMV, whereas UM130 (derived from Cabb S by the insertion of a short oligonucleotide in open reading frame III) coexisted in turnip plants with D/H, NY8153, CM4-184 and W isolates. To determine whether there are additional sequences responsible for the ability of Cabb S to dominate over UM130, co-inoculation experiments were done using chimeric DNAs, obtained by recombination (IC141, IC143 and VR246) and by construction (W/CS and CS/W). Chimeras IC141, IC143 and VR246 coexisted in turnip plants with UM130. Chimera CS/W dominated over UM 130, whereas UM 130 dominated over W/CS. The location of Cabb S sequences in these chimeras suggests that more than one region is responsible for the competitive advantage of Cabb S DNA. A different modification of Cabb S DNA in open reading frame III also destroyed the ability of the DNA to dominate over the W isolate. In DNA from virions obtained after co-infection with UM130 and CS/W, more molecules were recovered with U M 130-specific sequences at position 1364 than with those at position 2040. This and similar results with progeny from W/CS and UM 130 co-infection suggest that genetic exchanges had occurred. However, exchanges were not detected in progeny from other mixed infections. Thus the dominance of the Cabb S isolate in mixed infections was due primarily to a better competitive ability of the Cabb S DNA. Plants inoculated first with UM130 and 2 to 8 days later with Cabb S contained only UM130 CaMV DNA, indicating that dominance was unable to overcome cross-protection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CaMV , competition and turnip plants
© Society for General Microbiology 1989
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-12-3427
1989-12-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/12/JV0700123427.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-12-3427&mimeType=html&fmt=ahah

References

  1. Armour S. L., Melcher U., Pirone T. P., Lyttle D. J., Essenberg R. C. 1983; Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30
     [Google Scholar]
  2. Balazs E., Guilley H., Jonard G., Richards K. 1982; Nucleotide sequence of DNA from an altered-virulence isolate D/H of the cauliflower mosaic virus. Gene 19:239–249
     [Google Scholar]
  3. Baughman G. A., Jacobs J. D., Howell S. H. 1988; Cauliflower mosaic virus gene VI produces a symptomatic phenotype in transgenic tobacco plants. Proceedings of the National Academy of SciencesU.S.A 85:733–737
     [Google Scholar]
  4. Choe I. S., Melcher U., Richards K., Lebeurier G., Essenberg R. C. 1985; Recombination between mutant cauliflower mosaic virus DNAs. Plant Molecular Biology 5:281–289
     [Google Scholar]
  5. Daubert S. D., Schoelz J., Debao L., Shepherd R. J. 1984; Expression of disease symptoms in cauliflower mosaic virus genomic hybrids. Journal of Molecular and Applied Genetics 2:537–547
     [Google Scholar]
  6. Dixon L., Nyffenegger T., Delley G., Martinez-Izquierdo J., Hohn T. 1986; Evidence for replicative recombination in cauliflower mosaic virus. Virology 150:463–468
     [Google Scholar]
  7. Franck A., Guilley H., Jonard G., Richards K., Hirth L. 1980; Nucleotide sequence of cauliflower mosaic virus DNA. Cell 21:285–294
     [Google Scholar]
  8. Gardner R. C., Shepherd R. J. 1980; A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA. Virology 106:159–161
     [Google Scholar]
  9. Grimsley N., Hohn T., Hohn B. 1986; Recombination in a plant virus: template-switching in cauliflower mosaic virus. EMBO Journal 5:641–646
     [Google Scholar]
  10. Howarth A. J., Gardner R. C., Messing J., Shepherd R. J. 1981; Nucleotide sequence of naturally occurring deletion mutants of cauliflower mosaic virus. Virology 112:678–685
     [Google Scholar]
  11. Howell S. H., Walker L. L., Dudley R. K. 1980; Cloned cauliflower mosaic virus DNA infects turnips (Brassica rapa). Science 208:1265–1267
     [Google Scholar]
  12. Howell S. H., Walker L. L., Walden R. M. 1981; Rescue of in vitro generated mutants of cloned cauliflower mosaic virus genome in infected plants. Nature, London 293:483–486
     [Google Scholar]
  13. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
     [Google Scholar]
  14. Lebeurier G., Hirth L., Hohn B., Hohn T. 1982; In vivo recombination of cauliflower mosaic virus DNA. Proceedings of the National Academy of SciencesU.S.A 79:2932–2936
     [Google Scholar]
  15. Mason W. S., Taylor J. M., Hull R. 1987; Retroid virus genome replication. Advances in Virus Research 32:35–96
     [Google Scholar]
  16. Melcher U. 1989; Symptoms of cauliflower mosaic virus infection in Arabidopsis thaliana and turnip. Botanical Gazette 150:139–147
     [Google Scholar]
  17. Melcher U., Gardner C. O. Jr, Essenberg R. C. 1981; Clones of cauliflower mosaic virus identified by molecular hybridization in turnip leaves. Plant Molecular Biology 1:63–73
     [Google Scholar]
  18. Melcher U., Choe I. S., Lebeurier G., Richards K., Essenberg R. C. 1986a; Selective allele loss and interference between cauliflower mosaic virus DNAs. Molecular and General Genetics 203:230–236
     [Google Scholar]
  19. Melcher U., Steffens D. L., Lyttle D. J., Lebeurier G., Lin H., Choe I. S., Essenberg R. C. 1986b; Infectious and non-infectious mutants of cauliflower mosaic virus DNA. Journal of General Virology 67:1491–1498
     [Google Scholar]
  20. Schoelz J., Shepherd R. J., Daubert S. 1986; Region VI of cauliflower mosaic virus encodes a host range determinant. Molecular and Cellular Biology 6:2632–2637
     [Google Scholar]
  21. Sun T. J., Melcher U., Essenberg M. 1988; Inactivation of cauliflower mosaic virus by a photoactivatable cotton phytoalexin. Physiological and Molecular Plant Pathology 33:115–126
     [Google Scholar]
  22. Tomlinson J. A., Shepherd R. J. 1978; Studies on mutagenesis and cross-protection of cauliflower mosaic virus. Annals of Applied Biology 90:223–231
     [Google Scholar]
  23. Urban L. A., Sherwood I. L., Rezende J. A. M., Melcher U. 1989; Examination of mechanisms of cross-protection with non-transgenic plants. NATO Advanced Study Institute in press
    [Google Scholar]
  24. Walden R. M., Howell S. H. 1982; Intergenomic recombination events among pairs of defective cauliflower mosaic virus genomes in plants. Journal of Molecular and Applied Genetics 1:447–456
     [Google Scholar]
  25. Walden R. M., Howell S. H. 1983; Uncut recombinant plasmids bearing nested cauliflower mosaic virus genomes infect plants by intragenomic recombination. Plant Molecular Biology 2:27–31
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-12-3427
Loading
Competition between Isolates and Variants of Cauliflower Mosaic Virus in Infected Turnip Plants
J. Gen. Virol. 70, 3427 (1989); https://doi.org/10.1099/0022-1317-70-12-3427
/content/journal/jgv/10.1099/0022-1317-70-12-3427
/content/journal/jgv/10.1099/0022-1317-70-12-3427
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error