1887

Abstract

SUMMARY

The nucleotide sequence of the genomic RNA of narcissus mosaic virus (NMV) was deduced from a set of cDNA clones and by direct sequencing of RNA. The genome, with a length of 6955 nucleotides [excluding the 3′poly(A) tail], contains six open reading frames (ORFs) with the capacity to code for polypeptides of more than 10K, with values of 186284, 25845, 13998, 11059, 26097 and 10519. The first five of these putative proteins show considerable homology to similar proteins encoded by the RNAs of potato virus X (PVX, 6435 nucleotides) and white clover mosaic virus (WClMV, 5845 nucleotides). The sixth ORF is completely overlapped by the 26097 coat protein cistron and has some homology with a similar ORF in WClMV RNA. The difference in length of the RNAs of NMV, PVX and WClMV is due to a non-homologous domain of variable length in the central region of the 5′-proximal ORF of the three viruses.

Keyword(s): NMV , nucleotide sequence and RNA
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-2-267
1989-02-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/2/JV0700020267.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-2-267&mimeType=html&fmt=ahah

References

  1. Abouhaidar M. 1981; Polar assembly of clover yellow mosaic virus. Journal of General Virology 57:199–203
    [Google Scholar]
  2. Abouhaidar M. G. 1983; The structure of the 5′ and 3′ ends of clover yellow mosaic virus RNA. Canadian Journal of Microbiology 29:151–156
    [Google Scholar]
  3. Abouhaidar M. G. 1988; Nucleotide sequence of the capsid protein gene and 3′ non-coding region of papaya mosaic virus RNA. Journal of General Virology 69:219–226
    [Google Scholar]
  4. Abouhaidar M. G., Bancroft J. B. 1978; The initiation of papaya mosaic virus assembly. Virology 90:54–59
    [Google Scholar]
  5. Bendena W. G., Mackie G. A. 1986; Translational strategies in potexviruses: products encoded by clover yellow mosaic virus, foxtail mosaic virus, and viola mottle virus RNAs in vitro. Virology 153:220–229
    [Google Scholar]
  6. Bendena W. G., Bancroft J. B., Mackie G. A. 1987; Molecular cloning of clover yellow mosaic virus RNA: identification of coat protein coding sequences in vivo and in vitro. Virology 157:276–284
    [Google Scholar]
  7. Bouzoubaa S., Guilley H., Jonard G., Richards K., Putz C. 1985; Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. Journal of General Virology 66:1553–1564
    [Google Scholar]
  8. Bouzoubaa S., Ziegler V., Beck D., Guilley H., Richards K., Jonard G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA-2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  9. Bouzoubaa S., Quillet L., Guilley H., Jonard G., Richards K. 1987; Nucleotide sequence of beet necrotic yellow vein virus RNA-1. Journal of General Virology 68:615–626
    [Google Scholar]
  10. Bundin V. S., Vishnyakova O. A., Zakharyev V. M., Morozov S. Y., Atabekov J. G., Skryabin K. G. 1986; Comparative studies of potexvirus genomes: homology between the primary structures of coat protein genes. Dokladӯ Akademii nauk SSSR 290:728–733
    [Google Scholar]
  11. Cornelissen B. J. C., Van Vloten-Doting L. 1988; Molecular biology of plant RNA viruses In Cell Culture and Somatic Cell Genetics of Plants. 7 Molecular Biology of Plant Nuclear Genes Schell J., Vasil I. K. Orlando: Academic Press;
    [Google Scholar]
  12. Cornelissen B. J. C., Linthorst H. J. M., Brederode F. Th., Bol J. F. 1986; Analysis of the genomic structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2169
    [Google Scholar]
  13. Davies J. W., Stanley J., Van Kammen A. 1979; Sequence homology adjacent to the 3′ terminal poly(A) of cowpea mosaic virus RNAs. Nucleic Acids Research 7:493–500
    [Google Scholar]
  14. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  15. Dolja V. V., Grama D. P., Morozov S. Y., Atabekov J. G. 1987; Potato virus X-related single- and doublestranded RNAs. FEBS Letters 214:308–312
    [Google Scholar]
  16. Forster R. L. S., Bevan M. W., Harblson S. A., Gardner R. C. 1988; The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Research 16:291–303
    [Google Scholar]
  17. Fukuda M., Okada Y., Otsuki Y., Takebe I. 1980; The site of rod assembly on the RNA of a tomato and a cowpea strain of tobacco mosaic virus. Virology 101:493–502
    [Google Scholar]
  18. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  19. Guilford P. J., Forster R. L. S. 1986; Detection of polyadenylated subgenomic RNAs in leaves infected with the potexvirus daphne virus X. Journal of General Virology 67:83–90
    [Google Scholar]
  20. Gustafson G., Armour S. L. 1986; The complete nucleotide sequence of RNA β from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
    [Google Scholar]
  21. Gustafson G., Hunter B., Hanau R., Armour S. L., Jackson A. O. 1987; Nucleotide sequence and genetic organization of barley stripe mosaic virus RNAs. Virology 158:394–406
    [Google Scholar]
  22. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  23. Kieny M. P., Lathe R., Lecocq J. R. 1983; New versatile cloning and sequencing vectors based on bacteriophage M13. Gene 25:263–269
    [Google Scholar]
  24. Koenig R., Lesemann D. E. 1978; Potexvirus group. CMI/AAB Descriptions of Plant Viruses no. 200
    [Google Scholar]
  25. Lok S., Abouhaidar M. G. 1986; The nucleotide sequence of the 5′ end of papaya mosaic virus RNA: site of in vitro assembly initiation. Virology 153:289–296
    [Google Scholar]
  26. Mackie G. A., Bancroft J. B. 1986; The longer RNA species in narcissus mosaic virus encodes all viral functions. Virology 153:215–219
    [Google Scholar]
  27. Morozov S. Yu., Zakchariev V. M., Chernov B. K., Prasolov V. S., Kozlov Yu. V., Atabekov J. G., Skryabin K. G. 1983; The analysis of the primary structure and localization of the coat protein gene of the genomic RNA of potato virus X. Dokladÿ Akademii nauk SSSR 271:211–215
    [Google Scholar]
  28. Morozov S. Yu., Lukasheva L. I., Chernov B. K., Skryabin K. G., Atabekov J. G. 1987; Nucleotide sequence of the open reading frames adjacent of the coat protein cistron in potato virus X. FEBS Letters 213:438–442
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  30. Short M. N., Davies J. W. 1983; Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  31. Sonenberg N., Shatkin A. J., Ricciardl R. P., Rubin M., Goodman R. M. 1978; Analysis of the terminal structures of RNA from potato virus X. Nucleic Acids Research 5:2501–2521
    [Google Scholar]
  32. Wodnar-Filipowicz A., Skrzeczkowski L. J., Filipowicz W. 1980; Translation of potato virus X RNA into high molecular weight proteins. FEBS Letters 109:151–155
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-2-267
Loading
/content/journal/jgv/10.1099/0022-1317-70-2-267
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error