1887

Abstract

SUMMARY

Fourteen hybridoma cell lines secreting antibodies against the Semliki Forest virus nucleocapsid protein were established and employed for identification of conserved epitopes among 27 alphavirus types and subtypes. Using an antibody capture test, the antibodies were found to cross-react to variable degrees with alphaviruses belonging to the Semliki Forest, western encephalitis and eastern encephalitis complexes, as well as Middelburg and Ndumu. None of the antibodies reacted with either Venezuelan equine encephalitis or Barmah Forest virus. Due to their reactivity with Fort Morgan, Y62-33, Whataroa and chikungunya, the monoclonal antibodies were divided into six reactive types. Competition assays showed that the epitopes for all the types were either identical or clustered on a single domain of the nucleocapsid protein.

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1989-03-01
2024-04-19
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References

  1. Bell J. R., Strauss E. G., Strauss J. H. 1979; Purification and amino acid compositions of the structural proteins of Sindbis virus. Virology 97:287–294
    [Google Scholar]
  2. Bell J. R., Kinney R. M., Trent D. W., Strauss E. G., Strauss J. H. 1984; An evolutionary tree relating eight alphaviruses, based on amino-terminal sequences of their glycoproteins. Proceedings of the National Academy of SciencesU.S.A 814702–4706
    [Google Scholar]
  3. Bidwell D. E., Bartlett A., Voller A. 1977; Enzyme immunoassays for viral diseases. Journal of Infectious Diseases 136:274–278
    [Google Scholar]
  4. Boege U., Wengler G., Wengler G., Wittmann-Liebold B. 1981; Primary structures of the core proteins of the alphaviruses Semliki Forest virus and Sindbis virus. Virology 113:293–303
    [Google Scholar]
  5. Boorsma D. M., Streefkerk J. G., Boonstra J., Bloemraad M., Van Zaane D. 1979; Periodate or glutaraldehyde for preparing peroxidase conjugates? Their use in laboratory diagnosis. Journal of Immunological Methods 30:245
    [Google Scholar]
  6. Calisher C. H., Shope R. E., Brandt W., Casals I., Karabatsos N., Murphy F. A., Tesh R. B., Wiebe M. E. 1980; Proposed antigenic classification of registered arboviruses. I. Togaviridae, Alphavirus. Intermrology 14:229–232
    [Google Scholar]
  7. Calisher C. H., Karabatsos N., Lazuik J. S., Monath T. P., Wolff K. L. 1988; Reevaluation of the western equine encephalitis antigenic complex of alphaviruses (family Togaviridae) as determined by neutralization tests. American Journal of Tropical Medicine and Hygiene 38:447–452
    [Google Scholar]
  8. Chanas A. C., Gould E. A., Clegg J. C. S., Varma M. G. R. 1982; Monoclonal antibodies to Sindbis virus glycoprotein E1 can neutralize, enhance infectivity, and independently inhibit haemagglutination or haemolysis. Journal of General Virology 58:37–46
    [Google Scholar]
  9. Clegg J. C. S., Chanas A. C., Gould E. A. 1983; Conformational changes in Sindbis virus E1 glycoprotein induced by monoclonal antibody binding. Journal of General Virology 64:1121–1126
    [Google Scholar]
  10. Dalgarno L., Short N. J., Hardy C. M., Bell J. R., Strauss J. H., Marshall I. D. 1984; Characterization of Barmah forest virus: an alphavirus with some unusual properties. Virology 133:416–426
    [Google Scholar]
  11. Dalrymple J. M., Teramoto A. Y., Cardiff R. D., Russell P. K. 1972; Radioimmune precipitation of group A arboviruses. Journal of Immunology 109:426–433
    [Google Scholar]
  12. Dalrymple J. M., Vogel S. N., Teramoto A. Y., Russell P. K. 1973; Antigenic components of group A arbovirus virions. Journal of Virology 12:1034–1042
    [Google Scholar]
  13. Davis N. L., Pence D. F., Meyer W. I., Schmaljohn A. L., Johnston R. E. 1987; Alternative forms of a strain-specific neutralizing antigenic site on the Sindbis virus E2 glycoprotein. Virology 161:101–108
    [Google Scholar]
  14. Figueiredo L. T. M., Shope R. E. 1987; An enzyme immunoassay for dengue antibody using infected cultured mosquito cells as antigen. Journal of Virological Methods 17:191–198
    [Google Scholar]
  15. Hancock K., Tsang V. C. W. 1983; India ink staining of proteins on nitrocellulose paper. Analytical Biochemistry 133:157–162
    [Google Scholar]
  16. Heinz F. X., Berger R., Tuma W., Kunz C. 1983; A topological and functional model of epitopes on the structural glycoprotein of tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126:525–537
    [Google Scholar]
  17. Horzinek M., Maess J., Laufs R. 1971; Studies on the substructure of Togaviruses. II. Analysis of equine arteritis, rubella, bovine viral diarrhea, and hog cholera viruses. Archiv fur die gesamte Virusforschung 33:306–318
    [Google Scholar]
  18. Kinney R. M., Johnson B. J., Brown V. L., Trent D. W. 1986; Nucleotide sequence of the 26S mRNA of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins. Virology 152:400–413
    [Google Scholar]
  19. Köhler G., Howe S. C., Milstein C. 1976; Fusion between immunoglobulin secreting and non secreting myeloma cell lines. European Journal of Immunology 6:292–295
    [Google Scholar]
  20. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  21. Porterfield J. S. 1980; Antigenic characteristics and classification of Togaviridae. In The Togaviruses13–46 Schlesinger R. W. New York & London: Academic Press;
    [Google Scholar]
  22. Roehrig J. T., Corser J. A., Schlesinger M. J. 1980; Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus. Virology 101:41–49
    [Google Scholar]
  23. Roehrig J. T., Gorski D., Schlesinger M. J. 1982; Properties of monoclonal antibodies directed against the glycoproteins of Sindbis virus. Journal of General Virology 59:421–425
    [Google Scholar]
  24. Shope R. E. 1985; Alphaviruses. In Virology931–953 Fields B. N. New York: Raven Press;
    [Google Scholar]
  25. Smith C, Wolcott J. A., Wust C. J., Brown A. 1985; Detection of immunologically cross-reacting capsid protein of alphaviruses on the surfaces of infected L929 cells. Journal of Virology 53:198–204
    [Google Scholar]
  26. Stanley J., Cooper S. J., Griffin D. E. 1985; Alphavirus neurovirulence: monoclonal antibodies discriminating wild-type from neuroadapted Sindbis virus. Journal of Virology 56:110–119
    [Google Scholar]
  27. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of SciencesU.S.A 764350–4354
    [Google Scholar]
  28. Trent D. W., Grant J. A. 1980; A comparison of new world alphaviruses in the western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques. Journal of General Virology 47:261–282
    [Google Scholar]
  29. Westaway E. G., Brinton M. A., Gaidamovich S. YA., Horzinek M. C., Igarashi A., Kääriäjnen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. 1985; Togaviridae. Intervirology 24:125–139
    [Google Scholar]
  30. Yamamoto K., Hashimoto K., Chiba J., Simizu B. 1985; Properties of monoclonal antibodies against glycoproteins of western equine encephalitis virus. Journal of Virology 55:840–842
    [Google Scholar]
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