1887

Abstract

Summary

The analysis of replicative form (RF) DNA of Aleutian disease virus (ADV) by alkaline gel electrophoresis revealed that all RF DNA species segregate into DNA single strands which represent integral multiples of a genome equivalent. This demonstrates that as with other autonomous parvoviruses, the virion and complementary DNA strands are frequently linked by hairpin structures and that also, nicks are present at subterminal sites. Approximately 50 % of the 5′-terminal hairpins contain a subterminal nick whereas no nick is detectable in the 3′-terminal hairpin. This finding together with the presence of nicks in the 3′ palindrome sequence of the dimer RF DNA (D RF DNA) bridge fragment is the first experimental proof for the so far hypothetical substrate specificity of a nickase. A novel DNA structure was identified in the monomer (M) RF DNA population. This molecule, designated ‘monomer covalently closed linear RF DNA’ (Mccl RF DNA), consists of a continuous, self-complementary, circular polynucleotide chain of twice the genome length. It was directly visualized by electron microscopy that denatured ADV M RF DNA is a single-stranded circular molecule of twice the genome length with covalently closed terminal hairpins on either end. Alkaline gradient centrifugations, enzymic assays and electrophoretic techniques confirmed the proposed structure. Moreover, evidence was obtained that the D RF DNA species contains an analogous Dccl RF DNA. It is suggested that the newly described Mccl RF DNA form is an important intermediate common to the DNA replication of all autonomously replicating parvoviruses.

Keyword(s): ADV , hairpin and replicative form
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1989-05-01
2024-03-28
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References

  1. Alexandersen S., Bloom M. E. 1987; Studies on the sequential development of acute interstitial pneumonia caused by Aleutian disease virus in mink kits. Journal of Virology 61:81–86
    [Google Scholar]
  2. Astell C. R., Thomson M., Chow M. B., Ward D. C. 1983a; Structure and replication of minute virus of mice DNA. Cold Spring Harbor Symposia on Quantitative Biology 47:751–762
    [Google Scholar]
  3. Astell C. R., Thomson M., Merchlinsky M., Ward D. C. 1983b; The complete DNA sequence of minute virus of mice, an autonomous parvovirus. Nucleic Acids Research 11:999–1018
    [Google Scholar]
  4. Astell C. R., Chow M. B., Ward D. C. 1985; Sequence analysis of the termini of virion and replicative forms of minute virus of mice DNA suggests a modified rolling hairpin model for autonomous parvovirus DNA replication. Journal of Virology 54:171–177
    [Google Scholar]
  5. Baroudy B. M., Venkatesan S., Moss B. 1982; Incompletely base-paired flip-flop terminal loops link the two DNA strands of vaccinia virus genome into one uninterrupted polynucleotide chain. Cell 28:315–324
    [Google Scholar]
  6. Bloom M. E., Race R. E., Wolfinbarger J. B. 1980; Characterization of Aleutian disease virus as a parvovirus. Journal of Virology 35:836–843
    [Google Scholar]
  7. Bloom M. E., Mayer L. W., Garon C. F. 1983; Characterization of the Aleutian disease virus genome and its intracellular forms. Journal of Virology 45:977–984
    [Google Scholar]
  8. Bloom M. E., Alexandersen S., Perryman S., Lechner D., Wolfinbarger J. B. 1988; Nucleotide sequence and genomic organization of Aleutian mink disease parvovirus (ADV): sequence comparisons between a nonpathogenic and a pathogenic strain of ADV. Journal of Virology 62:2903–2915
    [Google Scholar]
  9. Bourguignon G. J., Tattersall P. J., Ward D. C. 1976; DNA of minute virus of mice: self-priming, nonpermuted, single stranded genome with a 5′-terminal hairpin duplex. Journal of Virology 20:290–306
    [Google Scholar]
  10. Bratosin S., Laub O., Tal J., Aloni Y. 1979; Mechanism for circularization of linear DNAs: circular parvovirus MVM DNA is formed by a “noose” sliding in a “lasso”-like DNA structure. Proceedings of the National Academy of SciencesU.S.A 76:4289–4293
    [Google Scholar]
  11. Carter B. J., Khoury G. 1975; Specific cleavage of adenovirus-associated virus DNA by restriction endonuclease R. EcoRI – characterization of cleavage products. Virology 63:523–538
    [Google Scholar]
  12. Cavalier-Smith T. 1974; Palindromic base sequences and replication of eukaryote chromosome ends. Nature, London 250:467–470
    [Google Scholar]
  13. Chow M., Bodnar J. W., Polvino-Bodnar M., Ward D. C. 1986; Identification and characterization of a protein covalently bound to DNA of minute virus of mice. Journal of Virology 57:1094–1104
    [Google Scholar]
  14. Cooke H. J. 1987; Telomeres of the human X and Y chromosomes. Development 101: supplement 101–106
    [Google Scholar]
  15. Cotmore S. F., Tattersall P. 1987; The autonomously replicating parvoviruses of vertebrates. Advances in Virus Research 33:91–174
    [Google Scholar]
  16. Cotmore S. F., Tattersall P. 1988; The NS-1 polypeptide of minute virus of mice is covalently attached to the 5′ terminus of duplex replicative form DNA and progeny single strands. Journal of Virology 62:851–860
    [Google Scholar]
  17. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  18. Favaloro J., Treisman R., Kamen R. 1980; Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods in Enzymology 65:718–749
    [Google Scholar]
  19. Haas L., Lochelt M., Kaaden O.-R. 1988; Detection of Aleutian disease virus DNA in tissues of naturally infected mink. Journal of General Virology 69:705–710
    [Google Scholar]
  20. Hauswirth W. W. 1984; Autonomous parvovirus DNA structure and replication. In The Parvoviruses129–152 Berns K. I. New York & London: Plenum Press;
    [Google Scholar]
  21. Inman R. B. 1966; A denaturation map of the lambda phage DNA molecule determined by electron microscopy. Journal of Molecular Biology 18:464–476
    [Google Scholar]
  22. Kiss I., Boros I., Udvardy A., Venetianer P., Delius H. 1980; RNA-polymerase binding at the promoters of the rRNA genes of Escherichia coli . Biochimica et biophysica acta 609:435–447
    [Google Scholar]
  23. Lakritz N., Foglesong P. D., Reddy M., Baum S., Hurwitz J., Bauer W. R. 1985; A vaccinia virus DNase preparation which cross-links superhelical DNA. Journal of Virology 53:935–943
    [Google Scholar]
  24. Löchelt M., Kaaden O.-R. 1987; Lymphotropic strain SL3 of Aleutian disease virus: identification of replicative form DNA, molecular cloning and expression of capsid-specific proteins. Journal of General Virology 68:1041–1048
    [Google Scholar]
  25. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Pogo B. G. T. 1980; Changes in parental vaccinia virus DNA after viral penetration into cells. Virology 101:520–524
    [Google Scholar]
  27. Porter D. D., Larsen A. E., Cox N. A., Porter H. G., Suffin S. C. 1977; Isolation of Aleutian disease virus of mink in cell culture. Intervirology 8:129–144
    [Google Scholar]
  28. Singer I. I., Rhode S. L. III 1977; Replication process of the parvovirus H-1. VII. Electron microscopy of replicative-form DNA synthesis. Journal of Virology 21:713–723
    [Google Scholar]
  29. Straus S. E., Sebring E. D., Rose J. A. 1976; Concatemers of alternating plus and minus strands are intermediates in adeno-associated virus DNA synthesis. Proceedings of the National Academy of SciencesU.S.A 73:742–746
    [Google Scholar]
  30. Stuber D., Bujard H. 1977; Electron microscopy of DNA: determination of absolute molecular weights and linear density. Molecular and General Genetics 154:299–303
    [Google Scholar]
  31. Tattersall P., Ward D. C. 1976; Rolling hairpin model for replication of parvovirus and linear chromosomal DNA. Nature, London 263:106–109
    [Google Scholar]
  32. Vinograd J., Lebowitz J. 1966; Physical and topological properties of circular DNA. Journal of General Physiology 49:103–125
    [Google Scholar]
  33. Ward D. C., Dadachanji D. K. 1978; Replication of minute-virus-of-mice DNA. In Replication of Mammalian Parvoviruses297–313 Ward D. C., Tattersall P. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  34. Wobbe C. R., Mitra S. 1985; Proteins tightly associated with the termini of replicative form DNA of Kilham rat virus, an autonomous parvovirus. Proceedings of the National Academy of SciencesU.S.A 82:8335–8339
    [Google Scholar]
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