f Junin Virus Monoclonal Antibodies: Characterization and Cross-reactivity with Other Arenaviruses
- Authors: Anthony Sanchez, Dominique Y. Pifat, Richard H. Kenyon, Clarence J. Peters, Joseph B. McCormick, Michael P. Kiley
- First Published Online: 01 May 1989, Journal of General Virology 70: 1125-1132, doi: 10.1099/0022-1317-70-5-1125
- Subject: Animal
- Issue Published:
Twenty-one monoclonal antibodies reactive with Junin virus structural proteins were produced and characterized. Using radioimmunoprecipitation and Western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. In radioimmunoprecipitation assays, glycoprotein-specific monoclonal antibodies reacted not only with the 35K structural glycoprotein, but also with what is presumed to be the glycoprotein precursor. Four of seven glycoprotein-specific antibodies neutralized Junin virus to high titres. Cross-reactivity with other arenaviruses was found to be restricted to nucleoprotein-specific monoclonal antibodies and occurred only with New World arenaviruses. Cross-reactivity also shows the Junin virus to be most closely related to Machupo and Tacaribe viruses.
© Society for General Microbiology 1989 | Published by the Microbiology Society
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