f Detection of Dengue 4 Virus Core Protein in the Nucleus. II. Antibody against Dengue 4 Core Protein Produced by a Recombinant Baculovirus Reacts with the Antigen in the Nucleus
- Authors: Yoshihiro Makino, Masayuki Tadano, Toshikazu Anzai, Shao-Ping Ma, Shinobu Yasuda, Toshihiko Fukunaga
- First Published Online: 01 June 1989, Journal of General Virology 70: 1417-1425, doi: 10.1099/0022-1317-70-6-1417
- Subject: Animal
- Issue Published:
The dengue 4 virus (DEN-4) core gene and part of the PreM genes were inserted into the baculovirus polyhedrin gene region. The recombinant baculovirus directed the synthesis of the DEN-4 core protein fused to a part of the polyhedrin protein (M r 25K), as determined by Western blot analysis using DEN-4 core monoclonal antibody. A mouse polyclonal antibody prepared against the DEN-4 core fusion protein showed antigenic reactivity with the authentic DEN-4 core protein (M r 15.5K) present in the nucleus as well as in the cytoplasm of DEN-4-infected Vero cells as demonstrated by the peroxidase-antiperoxidase staining method. This antibody did not react with cells infected with DEN-1, -2, -3 or Japanese encephalitis virus, or mock-infected cells.
© Society for General Microbiology 1989 | Published by the Microbiology Society
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