@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-6-1417, author = "Makino, Yoshihiro and Tadano, Masayuki and Anzai, Toshikazu and Ma, Shao-Ping and Yasuda, Shinobu and Fukunaga, Toshihiko", title = "Detection of Dengue 4 Virus Core Protein in the Nucleus. II. Antibody against Dengue 4 Core Protein Produced by a Recombinant Baculovirus Reacts with the Antigen in the Nucleus", journal= "Journal of General Virology", year = "1989", volume = "70", number = "6", pages = "1417-1425", doi = "https://doi.org/10.1099/0022-1317-70-6-1417", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-6-1417", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "baculovirus recombinant", keywords = "dengue core gene", keywords = "intranuclear DEN-4 antigen", abstract = "SUMMARY The dengue 4 virus (DEN-4) core gene and part of the PreM genes were inserted into the baculovirus polyhedrin gene region. The recombinant baculovirus directed the synthesis of the DEN-4 core protein fused to a part of the polyhedrin protein (M r 25K), as determined by Western blot analysis using DEN-4 core monoclonal antibody. A mouse polyclonal antibody prepared against the DEN-4 core fusion protein showed antigenic reactivity with the authentic DEN-4 core protein (M r 15ยท5K) present in the nucleus as well as in the cytoplasm of DEN-4-infected Vero cells as demonstrated by the peroxidase-antiperoxidase staining method. This antibody did not react with cells infected with DEN-1, -2, -3 or Japanese encephalitis virus, or mock-infected cells.", }