%0 Journal Article %A Makino, Yoshihiro %A Tadano, Masayuki %A Anzai, Toshikazu %A Ma, Shao-Ping %A Yasuda, Shinobu %A Fukunaga, Toshihiko %T Detection of Dengue 4 Virus Core Protein in the Nucleus. II. Antibody against Dengue 4 Core Protein Produced by a Recombinant Baculovirus Reacts with the Antigen in the Nucleus %D 1989 %J Journal of General Virology, %V 70 %N 6 %P 1417-1425 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-70-6-1417 %K baculovirus recombinant %K dengue core gene %K intranuclear DEN-4 antigen %I Microbiology Society, %X SUMMARY The dengue 4 virus (DEN-4) core gene and part of the PreM genes were inserted into the baculovirus polyhedrin gene region. The recombinant baculovirus directed the synthesis of the DEN-4 core protein fused to a part of the polyhedrin protein (M r 25K), as determined by Western blot analysis using DEN-4 core monoclonal antibody. A mouse polyclonal antibody prepared against the DEN-4 core fusion protein showed antigenic reactivity with the authentic DEN-4 core protein (M r 15ยท5K) present in the nucleus as well as in the cytoplasm of DEN-4-infected Vero cells as demonstrated by the peroxidase-antiperoxidase staining method. This antibody did not react with cells infected with DEN-1, -2, -3 or Japanese encephalitis virus, or mock-infected cells. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-6-1417