Detection of Dengue 4 Virus Core Protein in the Nucleus. II. Antibody against Dengue 4 Core Protein Produced by a Recombinant Baculovirus Reacts with the Antigen in the Nucleus Makino, Yoshihiro and Tadano, Masayuki and Anzai, Toshikazu and Ma, Shao-Ping and Yasuda, Shinobu and Fukunaga, Toshihiko,, 70, 1417-1425 (1989), doi = https://doi.org/10.1099/0022-1317-70-6-1417, publicationName = Microbiology Society, issn = 0022-1317, abstract= SUMMARY The dengue 4 virus (DEN-4) core gene and part of the PreM genes were inserted into the baculovirus polyhedrin gene region. The recombinant baculovirus directed the synthesis of the DEN-4 core protein fused to a part of the polyhedrin protein (M r 25K), as determined by Western blot analysis using DEN-4 core monoclonal antibody. A mouse polyclonal antibody prepared against the DEN-4 core fusion protein showed antigenic reactivity with the authentic DEN-4 core protein (M r 15ยท5K) present in the nucleus as well as in the cytoplasm of DEN-4-infected Vero cells as demonstrated by the peroxidase-antiperoxidase staining method. This antibody did not react with cells infected with DEN-1, -2, -3 or Japanese encephalitis virus, or mock-infected cells., language=, type=