1887

Abstract

SUMMARY

Adult rats were infected with bovine leukaemia virus (BLV). Inoculated rats persistently produced antibodies directed against viral structural proteins. No major pathogenesis in infected rats was found during 2 years of observation. It was possible to recover the virus from rat spleen several months after infection. A cell line, R(BLV), was established from rat spleen; this contained integrated BLV provirus. R(BLV) cells kept for over 80 passages produced viral particles with the properties of BLV. Provirus reintegration and/or amplification occurred in R(BLV) cells. The cell line was found to be tumorigenic in rats, and the virus produced was immunogenic. R(BLV) cells represent the first described BLV-producing rat cell line. Proven persistent infection with BLV indirectly suggests that rats can serve as a reservoir of BLV in nature.

Keyword(s): BLV , integration and provirus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-7-1929
1989-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/7/JV0700071929.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-7-1929&mimeType=html&fmt=ahah

References

  1. Altaner C., Ban J., Zajac V., Rossler H., Rosenthal S., Kettmann R., Burny A. 1985; Isolation and characterization of cell clones producing various amounts of bovine leukosis virus. Folia biologica, Praha 31:107–114
    [Google Scholar]
  2. Altaner C., Ban J., Altanerova V., Burny A., Kettmann R. 1987a; Bovine leukemia virus: isolation and characterization of nonproducer cell clones. Neoplasma 34:641–652
    [Google Scholar]
  3. Altaner C., Ban J., Altanerova V., Cerny L. 1987b; Experimental transmission of the bovine leukemia virus genome by cell contact. Folia biologica, Praha 33:40CM09
    [Google Scholar]
  4. Burny A., Cleuter Y., Kettmann R., Mammerickx M., Portetelle D., van den Broeke A., Willems L., Thomas R. 1987; Bovine leukaemia: facts and hypotheses derived from the study of an infectious cancer. Cancer Surveys 6:139–159
    [Google Scholar]
  5. Deschamps J., Kettmann R., Burny A. 1981; Experiments with cloned complete tumor-derived bovine leukemia virus information prove that the virus is totally exogenous to its target animal species. Journal of Virology 40:605–609
    [Google Scholar]
  6. Ferrer J. F., Cabradilla C. D. 1978; The phenomenon of polykaryocytosis induced by BLV in mixed cultures: specificity, mechanisms and application to the diagnosis of BLV infection in cattle. Annales de Recherches Vétérinaires 9:721–728
    [Google Scholar]
  7. Ferrer J. F., Diglio C. A. 1976; Development of an in vitro infectivity assay for the C-type bovine leukemia virus. Cancer Research 36:1066–1073
    [Google Scholar]
  8. Ghysdael J., Bruck C., Kettmann R., Burny A. 1985; Bovine leukemia virus. Current Bottomics in Microbiology and Immunology 112:1–19
    [Google Scholar]
  9. Graves D. C., Ferrer J. F. 1976; In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Research 36:4152–4159
    [Google Scholar]
  10. Mammerickx M., Portetelle D., de Clercq K., Burny A. 1987; Experimental transmission of enzootic bovine leukosis to cattle, sheep and goats: infectious doses of blood and incubation period of the disease. Leukemia Research 11:353–358
    [Google Scholar]
  11. Olson C, Miller L. D., Miller J. M., Hoss H. E. 1972; Transmission of lymphosarcoma from cattle to sheep. Journal of the National Cancer Institute 49:1463–1467
    [Google Scholar]
  12. Olson O., Kettmann R., Burny A., Kaja R. 1981; Goat lymphosarcoma from bovine leukemia virus. Journal of the National Cancer Institute 67:671–675
    [Google Scholar]
  13. Oroszlan S., Sarngadharan M. G., Copeland T. D., Kalyanarman V. S., Gilden R. V., Gallo R. C. 1982; Primary structure analysis of the major internal p24 of human type C T-cell leukemia virus. Proceedings of the National Academy of SciencesU.S.A 79:1291–1294
    [Google Scholar]
  14. Shaw G. M., Gonda M. A., FlickinGER G. H., Hahn B. H., Gallo R. C., Wong-Staal F. 1984; Genomes of evolutionarily divergent members of the human T-cell leukemia virus family (HTLV-I and HTLV-II) are highly conserved, especially in pX. Proceedings of the National Academy of SciencesU.S.A 81:4544–4548
    [Google Scholar]
  15. Van Der maaten M. J., Miller J. M. 1976; Replication of bovine leukemia virus in monolayer cell cultures. Bibliotheca haematologica 43:360–362
    [Google Scholar]
  16. Zavada J., Cerny L., Zavadova Z., Bozonova J., Altstein A. D. 1979; A rapid neutralization test for antibodies to bovine leukemia virus, with the use of rhabdovirus pseudotypes. Journal of the National Cancer Institute 62:95–101
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-7-1929
Loading
/content/journal/jgv/10.1099/0022-1317-70-7-1929
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error