1887

Abstract

Nuclear run-on assays carried out in the presence and absence of the RNA polymerase II inhibitor, -amanitin, were used to determine the exact timing of the switch from inhibitor-sensitive transcription catalysed by host RNA polymerase II, to inhibitor-resistant transcription catalysed by the baculovirus-induced RNA polymerase. These studies revealed that the onset of -amanitin-resistant transcription is just after 6 h post-infection, simultaneous with the beginning of the late phase of infection. They also showed that transcripts from the p26 gene in the dIII Q/P region and the p35 gene in the dIII K/Q region of the viral genome are synthesized by the host RNA polymerase II both early and late in infection. On the other hand, transcripts of the pl0 gene in the dIII Q/P region and the transcripts in the dIII K region are synthesized by the -amanitin-resistant, virus-induced RNA polymerase late in infection.

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1990-01-01
2024-04-24
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