1887

Abstract

We have mapped a set of transcripts that cross the I site in the dIII F fragment of the genome of the nuclear polyhedrosis virus. These transcripts overlap at their 5′ ends by about 550 bases and run in opposite directions. We have tentatively identified two open reading frames corresponding to the leftward transcript. The rightward transcript is present from 8 h post-infection (p.i.) to 24 h p.i.; the leftward transcripts are present from 2 h p.i. to 24 h p.i. The early (2 h) transcript is about 2·1 kb in size and its 5′ end maps about 504 bp to the right of the I site. Beginning at about 8 h p.i. a new transcription start site is used, about 80 bp downstream (to the left) of the first. The late (rightward) transcript is about 1·2 kb in size; its 5′ end seems to be heterogeneous and maps about 44 to 60 bp to the left of the I site. Late in infection transcription proceeds in both directions at the same time through the overlapping region of the DNA encoding these transcripts.

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1990-01-01
2024-04-20
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References

  1. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  2. Blanton R. A., Carter T. H. 1979; Autoregulation of adenovirus type 5 early gene expression. III. Transcription studies in isolated nuclei. Journal of Virology 29:458–465
    [Google Scholar]
  3. Friesen P. D., Miller L. K. 1985; Temporal regulation of baculovirus RNA: overlapping early and late transcripts. Virology 54:392–400
    [Google Scholar]
  4. Friesen P. D., Miller L. K. 1987; Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:2264–2272
    [Google Scholar]
  5. Friesen P. D., Rice W. C., Miller D. W., Miller L. K. 1986; Bidirectional transcription from a solo long terminal repeat of the retrotransposon TED: symmetrical RNA start sites. Molecular and Cellular Biology 6:1599–1607
    [Google Scholar]
  6. Fuchs L. Y., Woods M. S., Weaver R. F. 1983; Viral transcription during Autographa califomica nuclear polyhedrosis virus infection: a novel RNA polymerase induced in infected Spodoptera frugiperda cells. Journal of Virology 48:641–646
    [Google Scholar]
  7. Gordon J. D., Carstens E. B. 1984; Phenotypic characterization and physical mapping of a temperature-sensitive mutant of Autographa califomica nuclear polyhedrosis virus defective in DNA synthesis. Virology 138:69–81
    [Google Scholar]
  8. Grula M., Buller P. L., Weaver R. F. 1981; α-amanitin-resistant viral RNA synthesis in nuclei isolated from nuclear polyhedrosis virus-infected Heliothis zea larvae and Spodoptera frugiperda cells. Journal of Virology 38:916–921
    [Google Scholar]
  9. Guarino L. A., Summers M. D. 1986; Functional mapping of a trans-activating gene required for expression of baculovirus delayed-early gene. Journal of Virology 57:563–571
    [Google Scholar]
  10. Kelly D. C., Lescott T. 1981; Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Microbiologica 4:35–47
    [Google Scholar]
  11. Kuzio J., Rohel D. Z., Curry C. J., Krebs A., Carstens E. B., Faulkner P. 1984; Nucleotide sequence of the p10 polypeptide gene of Autographa califomica nuclear polyhedrosis virus. Virology 139:414–418
    [Google Scholar]
  12. Lübbert H., Doerfler W. 1984; Transcription of overlapping sets of RNAs from the genome of Autographa califomica nuclear polyhedrosis virus: a novel method for mapping RNAs. Journal of Virology 52:255–265
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Matsuura Y., Possee R. D., Overton H. A., Bishop D. H. L. 1987; Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins. Journal of General Virology 68:1233–1250
    [Google Scholar]
  15. Miller L. K., Trimarchi R. E., Browne D., Pennock G. D. 1983; A temperature-sensitive mutant of the baculovirus Autographa califomica nuclear polyhedrosis virus defective in an early function required for further gene expression. Virology 126:376–380
    [Google Scholar]
  16. Oellig C., Happ B., Müller T., Doerfler W. 1987; Overlapping sets of viral RNAs reflect array of polypeptides in the EcoRI-J and -N fragments (map positions 81·2 to 85·0) of the Autographa califomica nuclear polyhedrosis virus genome. Journal of Virology 61:3048–3057
    [Google Scholar]
  17. Osborne T. F., Arvidson D. N., Tyau E. S., Dunsworthe-Browne M., Berk A. J. 1984; Transcription control region within the protein-coding portion of adenovirus EIA genes. Molecular and Cellular Biology 4:1293–1305
    [Google Scholar]
  18. Possee R. D., Howard S. C. 1987; Analysis of the polyhedrin gene promoter of the Autographa califomica nuclear polyhedrosis virus. Nucleic Acids Research 15:10233–10248
    [Google Scholar]
  19. Qin J., Liu A., Weaver R. F. 1989; Studies on the control region of the plO gene of the Autographa califomica nuclear polyhedrosis virus. Journal of General Virology 70:1273–1279
    [Google Scholar]
  20. Rankin C. B., Ladin B. F., Weaver R. F. 1986; Physical mapping of temporally regulated, overlapping transcripts in the region of the 10K protein gene in Autographa califomica nuclear polyhedrosis virus. Journal of Virology 57:18–27
    [Google Scholar]
  21. Rankin C., Ooi B. G., Miller L. K. 1988; Eight base pairs encompassing the transcriptional start point are the major determinant for baculoviruspolyhedrin gene expression. Gene 70:39–49
    [Google Scholar]
  22. Weaver R. F., Weissmann C. 1979; Mapping of an RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15S β-globin mRNA precursor and mature β-globm mRNA have identical map coordinates. Nucleic Acids Research 7:1175–1193
    [Google Scholar]
  23. Weyer U., Possee R. D. 1988; Functional analysis of the plO gene 5′ leader sequence of the Autographa califomica nuclear polyhedrosis virus. Nucleic Acids Research 16:3635–3653
    [Google Scholar]
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