f Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells
- Authors: M. D. Parker, D. Yoo, G. J. Cox, L. A. Babiuk
- First Published Online: 01 February 1990, Journal of General Virology 71: 263-270, doi: 10.1099/0022-1317-71-2-263
- Subject: Animal
- Published Online:
The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant of Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.
© Society for General Microbiology 1990 | Published by the Microbiology Society
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