%0 Journal Article %A Parker, M. D. %A Yoo, D. %A Cox, G. J. %A Babiuk, L. A. %T Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells %D 1990 %J Journal of General Virology, %V 71 %N 2 %P 263-270 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-71-2-263 %I Microbiology Society, %X The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant of Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-2-263