f Mutations of the human papillomavirus type 16 E7 gene that affect transformation, transactivation and phosphorylation by the E7 protein
- Authors: Alan Storey, Neil Almond†, Kit Osborn‡>, Lionel Crawford
- First Published Online: 01 April 1990, Journal of General Virology 71: 965-970, doi: 10.1099/0022-1317-71-4-965
- Subject: Animal
- Issue Published:
The human papillomavirus type 16 (HPV-16) E7 gene cooperates with an activated ras oncogene to transform primary rodent cells and is important in the immortalization of cervical keratinocytes. We have generated a series of point mutations within the E7 gene and show that mutation of residues serine 31 and serine 71 affect the phosphorylation of the E7 protein, but do not alter its ability to cooperate with ras. Further mutations which alter cysteine residues in a -Cys-X-X-Cys- motif decrease transformation markedly, although they do not abolish it entirely. All the mutations generated displayed a decreased ability to transactivate the adenovirus E2 promoter. These results show that neither phosphorylation of E7 nor its ability to transactivate are required for transformation by E7.
Present address: National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, U.K.
Present address: Ludwig Institute for Cancer Research, St Mary's Hospital Medical School, Norfolk Place, London W2 1PG, U.K.
© Society for General Microbiology 1990 | Published by the Microbiology Society
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