1887

Abstract

A region of the baculovirus nuclear polyhedrosis virus genome that is frequently found to be altered after serial passage of the virus in cell culture was characterized. Sequence analysis of this region of the genome in wild-type and mutant viruses revealed that some of the mutations affected a 675 bp open reading frame, designated DA26. The DA26 gene was disrupted both by deletion and by insertion of sequences that resembled transposable elements. Northern blot analysis of DA26 showed that it was expressed very early after infection. DA26-specific transcripts could be detected after the 1 h viral adsorption period upon infection of cultured cells. These transcripts were mapped by nuclease protection assays. A recombinant virus was constructed in which DA26 was disrupted by insertion of the gene. This virus was viable in both and cells and analysis of the kinetics of protein synthesis revealed no differences between wild-type and recombinant viruses. The disruption of DA26 also did not interfere with the ability of the virus to infect or larvae.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-5-1029
1990-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/5/JV0710051029.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-5-1029&mimeType=html&fmt=ahah

References

  1. Beames B., Summers M. D. 1988; Comparisons of host cell DNA insertions and altered transcription at the site of insertions in few polyhedra baculovirus mutants. Virology 162:206–220
    [Google Scholar]
  2. Beames B., Summers M. D. 1989; Location and nucleotide sequence of the 25K protein missing from baculovirus few polyhedra mutants. Virology 168:344–353
    [Google Scholar]
  3. Burand J. P., Summers M. D. 1982; Alteration of Autographa californicanuclear polyhedrosis virus DNA upon serial passage in cell culture. Virology 119:223–229
    [Google Scholar]
  4. Burton R. L. 1969; Mass rearing the corn earworm in the laboratory. USDA-ARS (Series) 33:134
    [Google Scholar]
  5. Carstens E. B. 1987; Identification and nucleotide sequence of the region of the AcMNPV genome carrying insertion elements from Spodoptera frugiperda . Virology 161:8–17
    [Google Scholar]
  6. Cary L. C., Goebel M., Corsaro B. G., Wang H.-G., Rosen E., Fraser M. J. 1989; Transposon mutagenesis of baculoviruses: analysis of Trichoplusia nitransposon IFP2 insertion within the FP locus of nuclear polyhedrosis virus. Virology 172:156–169
    [Google Scholar]
  7. Casadaban M. J., Martinez-Ariaz A., Shapiro S. K., Chow J. 1983; Beta-galactosidase gene fusions for analysing gene expression in E. coliand yeast. Methods in Enzymology 100:293–303
    [Google Scholar]
  8. Crawford A. M., Miller L. K. 1988; Characterization of an early gene accelerating expression of late genes of the baculovirus Autographa californicanuclear polyhedrosis virus. Journal of Virology 62:2773–2781
    [Google Scholar]
  9. Daum R. J. 1970; A revision of two computer programs for probit analysis. Bulletin of the Entomological Society of America 16:10–15
    [Google Scholar]
  10. Davis L. G., Dibner M. D., Battey J. F. 1986 Basic Methods in Molecular Biology New York: Elsevier;
    [Google Scholar]
  11. Fraser M. J., Hink W. F. 1982; The isolation and characterization of the MP and FP plaque variants of Galleria mellonellanuclear polyhedrosis virus. Virology 117:366–378
    [Google Scholar]
  12. Fraser M. J., Smith G. E., Summers M. D. 1983; Acquisition of host cell DNA sequences by baculoviruses: relationship between host DNA insertions and FP mutantsof Autographa californicaand Galleria mellonellanuclear polyhedrosis viruses. Journal of Virology 47:287–300
    [Google Scholar]
  13. Fraser M. J., Brusca J. S., Smith G. E., Summers M. D. 1985; Transposon-mediated mutagenesis of a baculovirus. Virology 145:352–361
    [Google Scholar]
  14. Guarino L. A., Summers M. D. 1988; Functional mapping of Autographa californicanuclear polyhedrosis virus genes required for late gene expression. Journal of Virology 62:463–471
    [Google Scholar]
  15. Hink W. F. 1970; Established insect cell line from the cabbage looper Trichoplusia ni . Nature; London: 225464–467
    [Google Scholar]
  16. Hink W. F., Vail P. V. 1973; A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN-368) cell line. Journal of Invertebrate Pathology 27:49–55
    [Google Scholar]
  17. Kumar S., Miller L. K. 1987; Effects of serial passage of Autographa californicanuclear polyhedrosis virus in cell culture. Virus Research 7:335–349
    [Google Scholar]
  18. Lee H. H., Miller L. K. 1978; Isolation of genotypic variants of Autographa californicanuclear polyhedrosis virus. Journal of Virology 27:754–767
    [Google Scholar]
  19. Miller D. W., Miller L. K. 1982; A virus mutant with an insertion of a copia-like transposable element. Nature; London: 299562–564
    [Google Scholar]
  20. Miller D. W., Safer P., Miller L. K. 1986; An insect baculovirus host-vector system for high level expression of foreign genes. In Genetic Engineering: Principles and Methods 8 pp Setlow J. K., Hollaender A. Edited by New York & London: Plenum Press;
    [Google Scholar]
  21. Miller L. K., Dawes K. P. 1978; Restriction endonuclease analysis for the identification of baculovirus pesticides. Applied and Environmental Microbiology 35:411–421
    [Google Scholar]
  22. Nissen M. S., Friesen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
    [Google Scholar]
  23. O’Reilly D. R., Miller L. K. 1988; Expression and complex formation of simian virus 40 large T antigen and mouse p53 in insect cells. Journal of Virology 62:3109–3119
    [Google Scholar]
  24. O’Reilly D. R., Miller L. K. 1989; A baculovirus blocks molting of its insect host by expressing an ecdysteroid UDP-glucosyl transferase gene. Science 245:1110–1112
    [Google Scholar]
  25. O’Reilly D. R., Miller L. K. 1990; Regulation of expression of a baculovirus ecdysteroid UDP-glucosyl transferase gene. Journal of Virology 64:1321–1328
    [Google Scholar]
  26. Pennock G. D., Shoemaker C., Miller L. K. 1984; Strong and regulated expression of E. coli β-galactosidase in insect cells with a baculovirus vector. Molecular and Cellular Biology 4:399–406
    [Google Scholar]
  27. Potter K. N., Miller L. K. 1980; Correlating genetic mutations of a baculovirus with the physical map of the DNA genome. In Animal Virus Genetics, ICN-UCLA Symposia on Molecular and Cellular Biology 18 pp 71–80 Fields B. N., Jaenisch R. R., Fox C. F. Edited by New York: Academic Press;
    [Google Scholar]
  28. Potter K. N., Faulkener P., MacKinnon E. A. 1976; Strain selection during serial passage of Trichoplusia ninuclear polyhedrosis virus. Journal of Virology 18:1040–1050
    [Google Scholar]
  29. Thiem S. M., Miller L. K. 1989; Identification, sequence and transcriptional mapping of the major capsid protein gene of the baculovirus Autographa californicanuclear polyhedrosis virus. Journal of Virology 63:2008–2018
    [Google Scholar]
  30. Tomalski M. D., Wu J., Miller L. K. 1988; The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene. Virology 167:591–600
    [Google Scholar]
  31. Treat T. L., Halfhill I. E. 1973; Rearing alfalfa loopers and celery loopers on an artificial diet. Journal of Economic Entomology 66:569–570
    [Google Scholar]
  32. Vaughn J. L., Goodwin R. H., Thompkins G. L., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda(Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  33. Wang H.-G., Fraser M. J., Cary L. C. 1989; Transposon mutagenesis of baculoviruses: analysis of TFP3 lepidopteran transposons at the FP locus of nuclear polyhedrosis virus. Gene 81:97–108
    [Google Scholar]
  34. Weaver R. F., Weissmann C. 1979; Mapping of RNA by a modification of the Berk-Sharp procedure: the 5ʹ termini of 15S betaglobin mRNA precursor and mature 10S beta-globin mRNA have identical map coordinates. Nucleic Acids Research 7:1175–1193
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-5-1029
Loading
/content/journal/jgv/10.1099/0022-1317-71-5-1029
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error