1887

Abstract

Blood-borne type non-A, non-B (NANB) hepatitis- associated microtubular aggregates protein was isolated and partially sequenced. The microtubular aggregates were isolated from the hepatocytes of NANB- infected chimpanzees and were found to have a buoyant density in sucrose solution of 1·21 to 1·23 g/ml. A single protein, recognized by our anti-microtubular aggregates monoclonal antibodies, was found to have an of 44000 (p44). This p44 protein was not found in uninfected chimpanzees. We determined a partial amino acid sequence for p44, and showed that it has no homology to any known proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-1999
1990-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710091999.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-1999&mimeType=html&fmt=ahah

References

  1. Alter H. J., Purcell R. H., Feinstone S. M., Tegtmeier G. E. 1982; Non-A, non-B hepatitis: its relationship to cytomegalovirus, to chronic hepatitis, and to direct and indirect test methods. In 1981 International Symposium on Viral Hepatitis pp. 279–294 Szmuness W., Alter H. J., Maynard J. E. Edited by Philadelphia: Franklin Institute Press;
    [Google Scholar]
  2. Bradley D. W., Maynard J. E. 1986; Etiology and natural history of post-transfusion and enterically-transmitted non-A, non-B hepatitis. Seminars in Liver Disease 6:56–66
    [Google Scholar]
  3. Bradley D. W., Maynard J. E., Cook E. H., Ebert J. W., Gravelle C. R., Tsiquaye K. N., Kessler H., Zuckerman A. J., Miller M. F., Ling C. -M., Overby L. R. 1980; Non-A, non-B hepatitis in experimentally infected chimpanzees: cross-challenge and electron microscopic studies. Journal of Medical Virology 6:185–201
    [Google Scholar]
  4. Bradley D. W., Maynard J. E., Popper H., Cook E. H., Ebert J. W., McCaustland K. A., Schable C. A., Fields H. A. 1983; Posttransfusion non-A, non-B hepatitis: physicochemical properties of two distinct agents. Journal of Infectious Diseases 148:254–265
    [Google Scholar]
  5. Bradley D. W., McCaustland K. A., Cook E. H., Schable C. A., Ebert J. W., Maynard J. E. 1985; Posttransfusion non-A, non-B hepatitis in chimpanzees. Gastroenterology 88:773–779
    [Google Scholar]
  6. Bradley D. W., Krawczynski K., Cook E. H. JR McCaustland K. A., Humpherey C. D., Spelbering J. E., Myint H., Maynard J. E. 1987; Enterically transmitted non-A, non-B hepatitis: serial passage of disease in cynomolgus macaques and tamarins and recovery of disease-associated 27- to 34-nm viruslike particles. Proceedings of the National Academy of Sciences, U.S.A 84:6277–6281
    [Google Scholar]
  7. Choo Q. -L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  8. Hewick R. M., Hunkapiller M. W., Hood L. E., Dreyer W. J. 1981; A gas-liquid solid phase peptide and protein sequenator. Journal of Biological Chemistry 256:7990–7997
    [Google Scholar]
  9. Hunkapiller M. W., Lujan E., Ostrander F., Hood L. E. 1983; Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis. Methods in Enzymology 91:227–236
    [Google Scholar]
  10. Khuroo M. S. 1980; Study of an epidemic of non-A, non-B hepatitis. American Journal of Medicine 68:818–824
    [Google Scholar]
  11. Korn L. J., Queen C. L., Wegman M. W. 1977; Computer analysis of nucleic acid regulatory sequences. Proceedings of the National Academy of Sciences, U.S.A 74:4401–4405
    [Google Scholar]
  12. Krawczynski K., Bradley D. W., Shimizu Y., Humpherey C. D., Nainan O. V., Margolis H. S., Maynard J. E. 1985; Immunomorphologic studies of antigen identified by a monoclonal antibody associated with experimental non-A, non-B hepatitis in chimpanzees. Hepatology 5:964 abstract 72
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. Maeda T., Honda Y., Hanawa M., Yamada E., Ono Y., Shikata T., Shimizu Y. K. 1989; Production of antibodies directed against microtubular aggregates in hepatocytes of chimpanzees with non-A, non-B hepatitis. Journal of General Virology 70:1401–1407
    [Google Scholar]
  16. Masaki T., Fujihashi T., Nakamura K., Soejima M. 1981; Studies on a new proteolytic enzyme from AchromobacterlyticusM497-1. II. Specificity and inhibition studies of Achromobacterprotease. I. Biochimica et biophysica acta 660:51–55
    [Google Scholar]
  17. Pfeifer U., Thomssen R., Legler K., Böttcher U., Gerlich W., Weinmann E., Klinge O. 1980; Experimental non-A, non-B hepatitis: four types of cytoplasmic alteration in hepatocytes of infected chimpanzees. VirchowsArchiv B [Cell Pathology] 33:233–243
    [Google Scholar]
  18. Reyes G. R., Purdy M. A., Kim J. P., Luk K. -C., Young L. M., Fry K. E., Bradley D. W. 1990; Isolation of a cDNA from the virus responsible for enterically transmitted non-A, non-B hepatitis. Science 247:1335–1339
    [Google Scholar]
  19. Sherlock S. 1986; Viral hepatitis. Digestive Disease and Sciences 31:122S–132S
    [Google Scholar]
  20. Shimizu Y. K., Feinstone S. M., Purcell R. H., Alter H. J., London W. T. 1979; Non-A, non-B hepatitis: ultrastructural evidence for two agents in experimentally infected chimpanzees. Science 205:197–200
    [Google Scholar]
  21. Shimizu Y. K., Oomura M., Abe K., Uno M., Yamada E., Ono Y., Shikata T. 1985; Production of antibody associated with non-A, non-B hepatitis in a chimpanzee lymphoblastoid cell line established by in vitro transformation with Epstein-Barr virus. Proceedings of the National Academy of Sciences, U.S.A 82:2138–2142
    [Google Scholar]
  22. Shimizu Y. K., Purcell R. H., Gerin J. L., Feinstone S. M., Ono Y., Shikata T. 1986; Further studies by immunofluorescence of the monoclonal antibodies associated with experimental non-A, non- B hepatitis in chimpanzees and their relation to D hepatitis. Hepatology 6:1329–1333
    [Google Scholar]
  23. Shimizu Y. K., Purcell R. H., Gerin J. L., Feinstone S. M., Ono Y., Shikata T. 1987; Relation between non-A, non-B and delta hepatitis: studies with monoclonal antibodies. In The Hepatitis Delta Virus and Its Infection pp. 51–60 Rizzetto M., Gerin J. L., Purcell R. H. Edited by New York: Alan R. Liss;
    [Google Scholar]
  24. Takahashi K., Kitamura N., Shibui T., Kamizono M., Matsui R., Yoshiyama Y., Maeda T., Kondo J., Honda Y., Yamada E., Shimizu Y. K., Teranishi Y., Nakanishi S. 1990; Cloning, sequencing and expression in Escherichia coli of cDNA for a non-A, non-B hepatitis-associated microtubular aggregates protein. Journal of General Virology 71:2005–2011
    [Google Scholar]
  25. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  26. Tsunasawa S., Kondo J., Sakiyama F. 1985; Isocratic separation of PTH-amino acids at picomole level by reverse-phase HPLC in the presence of sodium dodecylsulfate. Journal of Biochemistry 97:701–704
    [Google Scholar]
  27. Yoshizawa H., Itoh Y., Iwakiri S., Kitajima K., Tanaka A., Nojiri T., Miyakawa Y., Mayumi M. 1981; Demonstration of two different types of non-A, non-B hepatitis by reinjection and cross-challenge studies in chimpanzees. Gastroenterology 81:107–113
    [Google Scholar]
  28. Yoshizawa H., Itoh Y., Iwakiri S., Kitajima K., Tanaka A., Tachibana T., Nakamura T., Miyakawa Y., Mayumi M. 1982; Non-A, non-B (type 1) hepatitis agent capable of inducing tubular ultrastructures in the hepatocyte cytoplasm of chimpanzees: inactivation by formalin and heat. Gastroenterology 82:502–506
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-1999
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-1999
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error