f A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes
- Authors: Tonja M. Woodward, Barry R. Miller, Barry J. Beaty, Dennis W. Trent, John T. Roehrig
- First Published Online: 01 October 1991, Journal of General Virology 72: 2431-2435, doi: 10.1099/0022-1317-72-10-2431
- Subject: Animal
- Issue Published:
Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2h epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes.
© Society for General Microbiology 1991 | Published by the Microbiology Society
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