@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-10-2431, author = "Woodward, Tonja M. and Miller, Barry R. and Beaty, Barry J. and Trent, Dennis W. and Roehrig, John T.", title = "A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes", journal= "Journal of General Virology", year = "1991", volume = "72", number = "10", pages = "2431-2435", doi = "https://doi.org/10.1099/0022-1317-72-10-2431", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-10-2431", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2h epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes.", }