%0 Journal Article %A Woodward, Tonja M. %A Miller, Barry R. %A Beaty, Barry J. %A Trent, Dennis W. %A Roehrig, John T. %T A single amino acid change in the E2 glycoprotein of Venezuelan equine encephalitis virus affects replication and dissemination in Aedes aegypti mosquitoes %D 1991 %J Journal of General Virology, %V 72 %N 10 %P 2431-2435 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-72-10-2431 %I Microbiology Society, %X Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. In vitro experiments using an Aedes albopictus cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2h epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into Aedes aegypti mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in Ae. aegypti mosquitoes. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-10-2431