f Degenerate primers based on highly conserved regions of amino acid sequence in papillomaviruses can be used in a generalized polymerase chain reaction to detect productive human papillomavirus infections
- Authors: Peter J. F. Snijders, Chris J. L. M. Meijer, Jan M. M. Walboomers
- J. Gen. Virol., November 1991 72: 2781-2786, doi: 10.1099/0022-1317-72-11-2781
- Subject: Animal
- Published Online:
Degenerate primers based on highly conserved regions of amino acid sequence in papillomaviruses can be used in a generalized polymerase chain reaction to detect productive human papillomavirus infections, Page 1 of 1< Previous page | Next page > /docserver/preview/fulltext/jgv/72/11/JV0720112781-1.gif
Conserved amino acid sequences within the L1 open reading frame of the human papillomavirus (HPV) genome were used as a basis to design two degenerate primers (GP17 and GP18) and one general probe (GPR22) which direct polymerase chain reaction (PCR) amplification and subsequent detection of a 620 to 660 bp DNA fragment. The conserved nature of the primers and probe was tested experimentally on a panel of 24 cloned HPV DNAs isolated from cutaneous and mucosal lesions, including HPV-2a and -57, which are known to be associated with lesions at both anatomical sites. The sensitivity of this PCR test was at the level of genomic Southern blot analysis, indicating that HPV infections producing high copy numbers can be detected. Positive results were obtained with DNA extracted from clinical samples of genital and cutaneous origin.
© Society for General Microbiology 1991 | Published by the Microbiology Society
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