%0 Journal Article %A Horzinek, Marian %A Keldermans, Lian %A Stuurman, Thea %A Black, John %A Herrewegh, Arnold %A Sillekens, Peter %A Koolen, Marck %T Bovine Immunodeficiency Virus: Immunochemical Characterization and Serological Survey %D 1991 %J Journal of General Virology, %V 72 %N 12 %P 2923-2928 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-72-12-2923 %I Microbiology Society, %X Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with M r values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-12-2923