RT Journal Article SR Electronic(1) A1 Horzinek, Marian A1 Keldermans, Lian A1 Stuurman, Thea A1 Black, John A1 Herrewegh, Arnold A1 Sillekens, Peter A1 Koolen, MarckYR 1991 T1 Bovine Immunodeficiency Virus: Immunochemical Characterization and Serological Survey JF Journal of General Virology, VO 72 IS 12 SP 2923 OP 2928 DO https://doi.org/10.1099/0022-1317-72-12-2923 PB Microbiology Society, SN 1465-2099, AB Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with M r values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-12-2923