1887

Abstract

We have transfected the single-stranded DNA form of the simian virus 40 (SV40)-based shuttle vector pZ189 into CV1P simian cells. Although the strand used did not code for the T antigen, we observed its conversion to a double-stranded DNA form. We deduced that the replication step converting a circular single-stranded DNA to a double-stranded one is independent of the SV40 T antigen in simian cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-12-3091
1991-12-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/12/JV0720123091.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-12-3091&mimeType=html&fmt=ahah

References

  1. Auborn K. J., Markowitz R. B., Wang E., Yu Y. T., Prives C. 1988; Simian virus 40 T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences. Journal of Virology 62:2204–2208
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Daya-Grosjean L., James M. R., Drougard C., Sarasin A. 1987; An immortalized xeroderma pigmentosum, group C, cell line which replicates SV40 shuttle vectors. Mutation Research 183:185–196
    [Google Scholar]
  4. Gluzman Y. 1981; SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23:175–182
    [Google Scholar]
  5. Huschtscha L. I., Holliday R. 1983; Limited and unlimited growth of SV40-transformed cells from human diploid MRC-5 fibroblasts. Journal of Cell Science 63:77–99
    [Google Scholar]
  6. Madzak C., Sarasin A. 1991; Mutation spectrum following transfection of ultraviolet-irradiated single-stranded or doublestranded shuttle vector DNA into monkey cells. Journal of Molecular Biology 218:667–673
    [Google Scholar]
  7. Madzak C., Menck C. F. M., Armier J., Sarasin A. 1989; Analysis of single-stranded DNA stability and damage-induced strand loss in mammalian cells using SV40-based shuttle vectors. Journal of Molecular Biology 205:501–509
    [Google Scholar]
  8. Sarasin A., Benoit A. 1980; Induction of an error-prone mode of DNA repair in UV-irradiated monkey kidney cells. Mutation Research 70:71–81
    [Google Scholar]
  9. Seidman M. M., Dixon K., Razzaque A., Zagursky R. J., Berman M. L. 1985; A shuttle vector plasmid for studying carcinogen-induced point mutations in mammalian cells. Gene 38:233–237
    [Google Scholar]
  10. Wilson J. H. 1978; Interference in SV40 DNA infections: a possible basis for cellular competence. Virology 91:380–388
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-12-3091
Loading
/content/journal/jgv/10.1099/0022-1317-72-12-3091
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error