1887

Abstract

A nucleotide sequence is reported for RNA-3, the smallest of the three major RNA species found in particles of raspberry bushy dwarf virus (RBDV). The sequence of 946 nucleotides contains a single large open reading frame which encodes an 30509 polypeptide. translation of RNA-3 yielded an 30000 product that reacted specifically with antiserum to RBDV particles and we conclude that the amino acid sequence deduced from the sequence of RNA-3 is that of the RBDV coat protein, or an immediate precursor of it. No affinities were detected by comparing the nucleotide sequence of RNA-3 with the sequences of other plant viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-2-469
1991-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/2/JV0720020469.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-2-469&mimeType=html&fmt=ahah

References

  1. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435–449
    [Google Scholar]
  2. DeBorde D. C., Naeve C. W., Herlocher M. L., Maassab H. F. 1986; Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Analytical Biochemistry 157:275–282
    [Google Scholar]
  3. Devereux J., Haeberli T. P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  4. Fauquet C., Dejardin J., Thouvenel J. C. 1986a; Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. I. Multidimensional classification of plant viruses. Intervirology 25:1–13
    [Google Scholar]
  5. Fauquet C., Dejardin J., Thouvenel J. C. 1986b; Evidence that the amino acid composition of the particle proteins of plant viruses is characteristic of the virus group. II. Discriminant analysis according to structural biological and classification properties of plant viruses. Intervirology 25:190–200
    [Google Scholar]
  6. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Mayo M. A., Reddy D. V. R. 1985; Translation products of RNA from Indian peanut clump virus. Journal of General Virology 66:1347–1351
    [Google Scholar]
  10. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70:1037–1051
    [Google Scholar]
  11. Murant A. F. 1975; Some properties of the particles of raspberry bushy dwarf virus. Proceedings of the American Phytopathological Society 2:116–117
    [Google Scholar]
  12. Murant A. F. 1976; Raspberry bushy dwarf virus. CMI/AAB Descriptions of Plant Viruses No. 165.
    [Google Scholar]
  13. Murant A. F. 1987; Raspberry bushy dwarf. In Virus Diseases of Small Fruits United States Department of Agriculture, Agriculture Handbook No. 631 pp. 229–234 Edited by Converse R. H. Washington: United States Government Printing Office;
    [Google Scholar]
  14. Murant A. F., Chambers J., Jones A. T. 1974; Spread of raspberry bushy dwarf virus by pollination, its association with crumbly fruit, and problems of control. Annals of Applied Biology 77:271–281
    [Google Scholar]
  15. Murant A. F., Mayo M. A., Raschke J. H. 1986; Some biochemical properties of raspberry bushy dwarf virus. Acta Horticulturae 186:23–30
    [Google Scholar]
  16. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 74:5463–5467
    [Google Scholar]
  17. St Aden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  18. Staden R. 1984; Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Research 12:521–538
    [Google Scholar]
  19. Symons R. H. 1985; Viral genome structure. In The Plant Viruses vol 1 Polyhedral Virions with Tripartite Genomes. pp. 57–81 Edited by Francki R. I. B. New York & London: Plenum Press;
    [Google Scholar]
  20. Wilson T. M. A. 1985; Nucleocapsid disassembly and early gene expression by positive-strand RNA viruses. Journal of General Virology 66:1201–1207
    [Google Scholar]
  21. Zimmern D., Kaesberg P. 1978; 3′-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U,. S,. A 75:4257–4261
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-2-469
Loading
/content/journal/jgv/10.1099/0022-1317-72-2-469
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error