1887

Abstract

Monoclonal antibodies (MAbs) were produced against two African arenaviruses, Lassa virus and Mopeia virus. Competitive binding analysis of MAbs identified four antigenic sites on the nucleoprotein (NP), two on glycoprotein 1 (GP1) and six on glycoprotein 2 (GP2) of the Josiah strain of Lassa virus. 64 virus isolates from western, central and southern Africa were all consistently distinguishable by MAbs to certain epitopic sites on GP1, GP2 and NP viral proteins. Furthermore, MAbs to Lassa virus GP1 and NP uniformly distinguished viruses from the West African countries of Sierra Leone, Liberia and Guinea from those of Nigeria. GP2-directed MAbs to two African arenaviruses reacted broadly with South American arenaviruses demonstrating that an epitopic site on GP2 may be the most highly conserved antigen in the arenavirus group.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-3-549
1991-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/3/JV0720030549.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-3-549&mimeType=html&fmt=ahah

References

  1. Auperin D. D., Romanowski V., Galinski M., Bishop D. H. L. 1984; Sequencing studies of Pichinde arenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA. Journal of Virology 52:897–904
    [Google Scholar]
  2. Auperin D. D., Sasso D. R., McCormick J. B. 1986; Nucleotide sequence of the glycoprotein gene and intergenic region of the Lassa virus S genome RNA. Virology 154:155–167
    [Google Scholar]
  3. Buchmeier M. J., Parekh B. S. 1987; Protein structure and expression among arenaviruses. Current Topics in Microbiology and Immunology 133:41–57
    [Google Scholar]
  4. Buchmeier M. J., Lewicki H. A., Tomori O., Johnson K. M. 1980; Monoclonal antibodies to lymphocytic choriomeningitis virus react with pathogenic arenaviruses. Nature, London 288:4876–4877
    [Google Scholar]
  5. Buchmeier M. J., Lewicki H. A., Oyewale T., Oldstone M. B. A. 1981; Monoclonal antibodies to lymphocytic choriomeningitis and Pichinde viruses: generation, characterization, and cross-reactivity with other arenaviruses. Virology 113:73–85
    [Google Scholar]
  6. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  7. Casals J., Buckley S. M., Cedeno R. 1975; Antigenic properties of the arenaviruses. Bulletin of the World Health Organization 52:421–427
    [Google Scholar]
  8. Clegg J. C. S., Lloyd G. 1983; Structural and cell-associated proteins of Lassa virus. Journal of General Virology 64:1127–1136
    [Google Scholar]
  9. Compans R. W., Bishop D. H. L. 1985; Biochemistry of arenaviruses. Current Topics in Microbiology & Immunology 114:153–175
    [Google Scholar]
  10. Elliott L. H., McCormick J. B., Johnson K. M. 1982; Inactivation of Lassa, Marburg and Ebola viruses by gamma irradiation. Journal of Clinical Microbiology 16:704–708
    [Google Scholar]
  11. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgGl, IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  12. Gonzalez J. P., Buchmeier M. J., McCormick J. B., Elliott L. H., Mitchell S. W., Kiley M. P. 1984; Comparative analysis of several Lassa-like arenavirus isolates from Africa. In Molecular Biology of Negative Strand Viruses pp 201–208 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier;
    [Google Scholar]
  13. Howard C. R., Lewicki H., Allison L., Salter M., Buchmeier M. J. 1985; Properties and characterization of monoclonal antibodies to Tacaribe virus. Journal of General Virology 66:1383–1395
    [Google Scholar]
  14. Hufert F. T., Ludke W., Schmitz H. 1989; Epitope mapping of the Lassa virus nucleoprotein using monoclonal anti-nucleocapsid antibodies. Archives of Virology 106:201–212
    [Google Scholar]
  15. Johnson K. M., Elliott L. H., Heymann D. L. 1981; Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human sera surveys. Journal of Clinical Microbiology 5:527–529
    [Google Scholar]
  16. Johnson K. M., Wiebenga N. H., Mackenzie R. B., Runs M. L., Tauraso N. M., Shelokov A., Webb P. A., Justines G., Beye H. K. 1965; Virus isolation from human cases of hemorrhagic fever in Bolivia. Proceedings of the Society for Experimental Biology and Medicine 118:113–118
    [Google Scholar]
  17. Kiley M. P., Tomori O., Regnery R. L., Johnson K. M. 1980; Characterization of the arenaviruses Lassa and Mozambique. In Replication of Negative Strand Viruses pp 1–9 Edited by Bishop D. H. L., Compans R. W. New York: Elsevier;
    [Google Scholar]
  18. Kohler G., Milsten C. 1976; Derivation of specific antibody-producing tissue and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Murphy F. A., Webb P. A., Johnson K. M., Whitfield S. G. 1969; Morphological comparison of Machupo with lymphocytic choriomeningitis virus: basis for a new taxonomic group. Journal of Virology 4:535–541
    [Google Scholar]
  21. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody: a new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  22. Parekh B. S., Buchmeier M. J. 1986; Proteins of lymphocytic choriomeningitis virus: antigenic topography of the viral glycoproteins. Virology 153:168–178
    [Google Scholar]
  23. Rowe W. P., Pugh W. E., Webb P. A., Peters C. J. 1970; Serological relationship of the Tacaribe complex of viruses to lymphocytic choriomeningitis virus. Journal of Virology 5:289–292
    [Google Scholar]
  24. Sanchez A., Pifat D. Y., Kenyon R. H., Peters C. J., McCormick J. B., Kiley M. P. 1989; Junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses. Journal of General Virology 70:1125–1132
    [Google Scholar]
  25. Tsang V. C. W., Peralta J. M., Simons A. R. 1983; Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92:377–391
    [Google Scholar]
  26. Webb P. A., Johnson K. M., Mackenzie R. B. 1969; The measurement of specific antibodies in Bolivian hemorrhagic fever by neutralization of virus plaques. Proceedings of the Society for Experimental Biology and Medicine 130:1013–1019
    [Google Scholar]
  27. Weber E. L., Buchmeier M. J. 1988; Fine mapping of a peptide sequence containing an antigenic site conserved among arenaviruses. Virology 164:30–38
    [Google Scholar]
  28. Wulff H., Lange J. V. 1975; Indirect immunofluorescence for the diagnosis of Lassa fever infection. Bulletin of the World Health Organization 52:429–436
    [Google Scholar]
  29. Young P. R., Chanas A. C., Lee S. R., Gould E. A., Howard C. R. 1987; Localization of an arenavirus protein in the nuclei of infected cells. Journal of General Virology 68:2465–2470
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-3-549
Loading
/content/journal/jgv/10.1099/0022-1317-72-3-549
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error