1887

Abstract

Analysis of porcine transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) mRNA species indicated a deletion in mRNA 3 of PRCV. Polymerase chain reaction (PCR) was used to clone the 5′ end of mRNA 3 from PRCV for comparison with the equivalent region in TGEV. Small deletions were observed within and around the PRCV sequence equivalent to the putative open reading frame (ORF) ORF-3a identified in TGEV. The potential RNA polymerase-leader complex binding site (leader RNA binding site), ACTAAAC, found upstream of ORF-3a in TGEV, was absent from the PRCV genome but a potential site was found in the PRCV genome upstream of a gene equivalent to TGEV ORF-3b. PCR analysis, using primers corresponding to sequences within the ORF-3b gene and the leader RNA sequence, confirmed that the leader RNA binding site was upstream of a gene equivalent to TGEV ORF-3b on PRCV mRNA 3 but upstream of ORF-3a on TGEV mRNA 3. The presence of the new leader RNA binding site would be responsible for generating the smaller mRNA 3 species found in PRCV-infected cells.

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1991-03-01
2024-03-28
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References

  1. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A. 80:3963–3965
    [Google Scholar]
  2. Britton P., Page K. W. 1990; Sequence of the S gene from a virulent British field isolate of transmissible gastroenteritis virus. Virus Research (in press)
    [Google Scholar]
  3. Britton P., Garwes D. J., Millson G. C., Page K., Bountiff L., Stewart F., Walmsley J. 1986; Towards a genetically-engineered vaccine against porcine transmissible gastroenteritis virus. In Biomolecular Engineering in the European Community (Final report) pp 301–313 Edited by Magnien E. The Hague: Martinus Nijhoff;
    [Google Scholar]
  4. Britton P., Garwes D. J., Page K., Walmsley J. 1987; Expression of porcine transmissible gastroenteritis virus genes in E. coli as β-galactosidase chimaeric proteins. In Coronaviruses, Advances in Experimental Medicine and Biology vol 218 pp 55–64 Edited by Lai M. M. C., Stohlman S. A. New York & London: Plenum Press;
    [Google Scholar]
  5. Britton P., Carmenes R. S., Page K. W., Garwes D. J., Parra F. 1988a; Sequence of the nucleoprotein from a virulent British field isolate of transmissible gastroenteritis virus and its expression in Saccharomyces cerevisiae . Molecular Microbiology 2:89–99
    [Google Scholar]
  6. Britton P., Carmenes R. S., Page K. W., Garwes D. J. 1988b; The integral membrane protein from a virulent isolate of transmissible gastroenteritis virus: molecular characterization, sequence and expression in Escherichia coli . Molecular Microbiology 2:497–505
    [Google Scholar]
  7. Britton P., Lopez Otin C., Martin Alonso J. M., Parra F. 1989; Sequence of the coding regions from the 3.0 kb and 3.9 kb mRNA subgenomic species from a virulent isolate of transmissible gastroenteritis virus. Archives of Virology 105:165–178
    [Google Scholar]
  8. Britton P., Garwes D. J., Page K., Stewart F. 1990; Molecular aspects of the relationship of transmissible gastroenteritis virus (TGEV) with porcine respiratory coronavirus (PRCV). In Coronaviruses and Their Diseases, Advances in Experimental Medicine and Biology vol 276 pp 441–446 Edited by Cavanagh D., Brown T. D. K. New York & London: Plenum Press;
    [Google Scholar]
  9. Brown I., Cartwright S. 1986; New porcine coronavirus?. Veterinary Record 119:282–283
    [Google Scholar]
  10. Cavanagh D., Brian D. A., Enjuanes L., Holmes K. V., Lai M. M. C., Laude H., Siddell S. G., Spaan W., Taguchi F., Talbot P. J. 1990; Recommendations of the coronavirus study group for the nomenclature of the structural proteins, mRNAs and genes of coronaviruses. Virology 176:306–307
    [Google Scholar]
  11. Cox E., Pensaert M. B., Callebaut P., Van Deun K. 1990; Intestinal implication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus. Veterinary Microbiology 23:237–243
    [Google Scholar]
  12. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  13. Doyle L. P., Hutchings L. M. 1946; A transmissible gastroenteritis in pigs. Journal of the American Veterinary Medical Association 108:257–259
    [Google Scholar]
  14. Garwes D. J., Pocock D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  15. Garwes D. J., Stewart F., Britton P. 1989; The polypeptide of M r 14000 of porcine transmissible gastroenteritis virus: gene assignment and intracellular location. Journal of General Virology 70:2495–2499
    [Google Scholar]
  16. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  17. Jacobs L., Van Der Zeijst B. A. M., Horzinek M. C. 1986; Characterization and translation of transmissible gastroenteritis virus mRNAs. Journal of Virology 57:1010–1015
    [Google Scholar]
  18. Jacobs L., de Groot R., van der Zeijst B. A. M., Horzinek M. C., Spaan W. 1987; The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (TGEV): comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (FIPV). Virus Research 8:363–371
    [Google Scholar]
  19. Kapke P. A., Brian D. A. 1986; Sequence analysis of the porcine transmissible gastroenteritis coronavirus nucleocapsid protein gene. Virology 151:41–49
    [Google Scholar]
  20. Kapke P. A., Tung F. Y. C., Brian D. A., Woods R. D., Wesley R. 1987; Nucleotide sequence of the porcine transmissible gastroenteritis coronavirus matrix protein. In Coronaviruses, Advances in Experimental Medicine and Biology vol 218 pp 117–122 Edited by Lai M. M. C., Stohlman S. A. New York & London: Plenum Press;
    [Google Scholar]
  21. Kapke P. A., Tung F. Y. T., Brian D. A. 1988; Nucleotide sequence between the peplomer and matrix protein genes of the porcine transmissible gastroenteritis coronavirus identifies three large open reading frames. Virus Genes 2:293–294
    [Google Scholar]
  22. Laude H., Rasschaert D., Huet J.-C. 1987; Sequence and N-terminal processing of the transmembrane protein El of the coronavirus transmissible gastroenteritis virus. Journal of General Virology 68:1687–1693
    [Google Scholar]
  23. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Murphy G., Kavanagh T. 1988; Speeding–up the sequencing of double–stranded DNA. Nucleic Acids Research 16:5198
    [Google Scholar]
  25. Page K. W., Britton P., Boursnell M. E. G. 1990; Sequence analysis of the leader RNA of two porcine coronaviruses: transmissible gastroenteritis virus and porcine respiratory coronavirus. Virus Genes 4:289–301
    [Google Scholar]
  26. Pensaert M. B. 1989; Transmissible gastroenteritis virus (respiratory variant). In Virus Infections of Vertebrates vol 2 pp 154–165 Edited by Horzinek M. C., Pensaert M. B. Amsterdam: Elsevier;
    [Google Scholar]
  27. Pensaert M. B., Callebaut P. E., Vergote J. 1986; Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Veterinary Quarterly 8:257–260
    [Google Scholar]
  28. Rasschaert D., Laude H. 1987; The predicted primary structure of the peplomer protein E2 of the porcine coronavirus transmissible gastroenteritis virus. Journal of General Virology 68:1883–1890
    [Google Scholar]
  29. Rasschaert D., Gelfi J., Laude H. 1987; Enteric coronavirus TGEV: partial sequence of the genomic RNA, its organisation and expression. Biochimie 69:591–600
    [Google Scholar]
  30. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi H., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  31. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  32. Underdahl N. R., Mebus C. A., Stair E. L., Rhodes M. B., McGill L. D., Twiehaus M. J. 1974; Isolation of transmissible gastroenteritis virus from lungs of market-weight swine. American Journal of Veterinary Research 35:1209–1216
    [Google Scholar]
  33. Wesley R. D., Cheung A. K., Michael D. D., Woods R. A. 1989; Nucleotide sequence of coronavirus TGEV genomic RNA: evidence for 3 mRNA species between the peplomer and matrix protein genes. Virus Research 13:87–100
    [Google Scholar]
  34. Winship P. R. 1989; An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Research 17:12–66
    [Google Scholar]
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