1887

Abstract

Two canine isolates of simian virus 5 (SV5), termed CPI+ and CPI-, were examined for their ability to react with a bank of monoclonal antibodies (MAbs) that had been previously raised against a human isolate of SV5. CPI-virus was originally isolated from the brain of a gnotobiotic dog infected with CPI+ virus and establishes persistent infections more readily than CPI+ . Of more than 50 MAbs tested, only one (P-k) reacted with CPI+ but not CPI-, enabling distinction between the two canine isolates. It had been shown previously that MAb P-k reacts with an epitope common to both the P and V proteins. In order to characterize further the epitope binding site of this MAb the P/V genes of CPI+ and CPI- were sequenced. There were four nucleotide differences between CPI+ and CPI-, three of which resulted in predicted amino acid substitutions. Synthetic peptides corresponding to regions encompassing these changes were made and radioimmune competition assays were used to identify the epitope binding site of MAb P-k. Sequence comparison of the P/V gene of CPI+ with the published sequence of a monkey isolate of SV5 (W3) revealed 14 nucleotide differences with five amino acid substitutions. The only amino acid substitution observed between CPI+, CPI- and W3 which altered the predicted secondary structures of the P and V proteins was a leucine to proline change that induced a predicted β-turn and resulted in the loss of binding of MAb P-k.

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1991-07-01
2024-03-29
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References

  1. Atherton E., Cameron L. R., Sheppard R. C. 1988; Peptide synthesis. Part 10. Use of pentafluorophenyl esters of fluorenyl methoxycarbonylamino acids in solid phase synthesis. Tetrahedron 44:843–857
    [Google Scholar]
  2. Baumgärtner W. K., Metzler A. E., Krakowka S., Koestner A. 1981; In vitro identification and characterisation of a virus isolated from a dog with neurological dysfunction. Infection and Immunity 31:1177–1183
    [Google Scholar]
  3. Baumgärtner W. K., Krakowka A., Koestner A., Evermann J. 1982; Acute encephalitis and hydrocephalus in dogs caused by canine parainfluenza virus. Vetinerary Pathology 19:79–92
    [Google Scholar]
  4. Baumgärtner W., Krakowka S., Blakeslee J. 1987; Evolution of in vitro persistence of two strains of canine parainfluenza virus. Archives of Virology 93:147–154
    [Google Scholar]
  5. Buetti E., Choppin P. W. 1977; The transcriptase complex of the paramyxovirus SV5. Virology 82:493–508
    [Google Scholar]
  6. Choppin P. W. 1964; Multiplication of a myxovirus (SV5) with minimal cytopathic effects and without interference. Virology 23:224–233
    [Google Scholar]
  7. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. Evermann J. F., Krakowka S., McKeirnan A. J., Baumgärtner W. 1981; Properties of an encephalitogenic canine parainfluenza virus. Archives of Virology 68:165–172
    [Google Scholar]
  10. Garnier J., Osguthorpe D. J., Robson B. 1978; Analysis of the accuracy and implications of simple methods for predicting secondary structure of proteins. Journal of Molecular Biology 120:97–120
    [Google Scholar]
  11. Goswami K. K. A., Lange L. S., Mitchell D. N., Cameron K. R., Russell W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
    [Google Scholar]
  12. Gough N. M. 1988; Rapid and quantitative preparation of cytoplasmic RNA from small numbers of cells. Analytical Biochemistry 173:93–95
    [Google Scholar]
  13. Hsiung G. D. 1972; Parainfluenza-5 virus. Infection of man and animals. Progress in Medical Virology 14:241–274
    [Google Scholar]
  14. Hull R., Minner J. R., Smith J. W. 1956; New viral agents recovered from tissue cultures of monkey cells. I. Origin and properties of cytopathogenic agents SV1, SV2, SV4, SV5, SV6, SV11, SV12 and SV15. American Journal of Hygiene 63:204–205
    [Google Scholar]
  15. Ito Y., Tsurudome M., Hishiyama M., Yamada A. 1987; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
    [Google Scholar]
  16. Jamerson B. A., Wolf H. 1988; The antigenic index – a novel algorithm for predicting antigenic determinants. Computer Applications in the Biosciences 4:181–186
    [Google Scholar]
  17. Kawano M., Bando H., Yuasa T., Kondo K., Tsurudome M., Komada H., Nishio M., Ito Y. 1990; Sequence determination of the hemagglutinin-neuraminidase (HN) gene of human parainfluenza type 2 virus and the construction of a phylogenetic tree for HN proteins of all the paramyxoviruses that are infectious to humans. Virology 174:314–319
    [Google Scholar]
  18. Kawasaki E. S. 1990; Amplification of RNA. In PCR Protocols A Guide to Methods and Applications pp 21–27 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. New York & London: Academic Press;
    [Google Scholar]
  19. McCandlish L. A. P., Thompson H., Cornwell H. J. C., Wright N. G. 1978; A study of dogs with kennel cough. Veterinary Record 102:298–301
    [Google Scholar]
  20. McSharry J. J., Compans R. W., Lackland H., Choppin P. W. 1975; Isolation and characterisation of the nonglycosylated membrane protein and a nucleocapsid complex from the paramyxovirus SV5. Virology 67:365–374
    [Google Scholar]
  21. Paterson R. G., Harris J. R. T., Lamb R. A. 1984; Analysis and gene assignment of a paramyxovirus, simian virus 5. Virology 138:310–323
    [Google Scholar]
  22. Precious B., Southern J. A., Randall R. E. 1990; Sequence analysis of the HN gene of parainfluenza virus type 2. Journal of General Virology 71:1163–1168
    [Google Scholar]
  23. Randall R. E., Russell W. C. 1991; Paramyxovirus persistence, consequences for host and virus. In Paramyxoviruses Edited by Kingsbury D. W. New York: Plenum Press; (in press)
    [Google Scholar]
  24. Randall R. E., Young D. F. 1988; Comparison between parainfluenza virus type 2 and simian virus 5: monoclonal antibodies reveal major antigenic differences. Journal of General Virology 69:2051–2060
    [Google Scholar]
  25. Randall R. E., Newman C., Honess R. W. 1984; Isolation and characterisation of monoclonal antibodies to structural and non-structural herpesvirus saimiri polypeptides. Journal of Virology 52:872–883
    [Google Scholar]
  26. Randall R. E., Young D. F., Goswami K. K. A., Russell W. C. 1987; Isolation and characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. Journal of General Virology 68:2769–2780
    [Google Scholar]
  27. Robbins S. J., Wrzos H., Kline A. L., Tenser R. B., Rapp F. 1981; Rescue of a cytopathic paramyxovirus from peripheral blood leukocytes in subacute sclerosing panencephalitis. Journal of Infectious Diseases 143:396–402
    [Google Scholar]
  28. Rosenberg F. J., Lief F. S., Todd J. D., Rief J. S. 1971; Studies of canine respiratory viruses. I. Experimental infection of dogs with an SV-5 like canine parainfluenza agent. American Journal of Epidemiology 94:147–165
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  30. Scharf S. J. 1990; Cloning with PCR. In PCR Protocols A Guide to Methods and Applications pp 21–27 Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. New York & London: Academic Press;
    [Google Scholar]
  31. Southern J. A., Precious B., Randall R. E. 1990; Two nontemplated nucleotide additions are required to generate the P mRNA of parainfluenza type 2 since the RNA genome encodes protein V. Virology 177:388–390
    [Google Scholar]
  32. Staden R. 1982a; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  33. Staden R. 1982b; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  34. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  35. Tribe G. W. 1966; An investigation into the incidence, epidemiology and control of simian virus 5. British Journal of Experimental Pathology 47:472–479
    [Google Scholar]
  36. Webster A., Russell W. C., Kemp G. D. 1989; Characterization of the adenovirus protease: development and use of a specific peptide assay. Journal of General Virology 70:3215–3223
    [Google Scholar]
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