f Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells
- Authors: Jean Taylor-Wiedeman, J. G. P. Sissons, L. K. Borysiewicz†, J. H. Sinclair
- First Published Online: 01 September 1991, Journal of General Virology 72: 2059-2064, doi: 10.1099/0022-1317-72-9-2059
- Subject: Animal
- Issue Published:
We have used the nested polymerase chain reaction (PCR) combined with fluorescence-activated cell sorting to define sites of latency of human cytomegalovirus (HCMV) in the peripheral blood of healthy subjects. Peripheral blood mononuclear (PBM) cells were separated into T cell or non-T cell populations and monocytes, and were then analysed by PCR for the presence of HCMV DNA. In five of six seropositive subjects, HCMV was found predominantly in the non-T cell population. Further analysis suggested that the virus was present in adherent cells and CD14+ cells. In three of nine seronegative subjects we could demonstrate HCMV DNA, which we do not believe was due to contamination, reproducibly by PCR. In one of these seronegative subjects, HCMV DNA was present predominantly in the non-T cell fraction of PBM cells. No HCMV DNA was detectable in the remaining six seronegative subjects. We conclude that, within the PBM cells of normal asymptomatic seropositive and some seronegative subjects, HCMV is present predominantly in the monocyte fraction. In addition, the detection of HCMV sequences in seronegative subjects may indicate that infection with HCMV is more widespread than conventional seroepidemiology suggests.
Present address: Department of Medicine, University of Wales, College of Medicine, Heath Park, Cardiff CF4 4XN, U.K.
© Society for General Microbiology 1991 | Published by the Microbiology Society
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