f Tomato spotted wilt virus L RNA encodes a putative RNA polymerase
- Authors: Peter de Haan, Richard Kormelink, Renato de Oliveira Resende, Frank van Poelwijk, Dick Peters, Rob Goldbach
- First Published Online: 01 September 1991, Journal of General Virology 72: 2207-2216, doi: 10.1099/0022-1317-72-9-2207
- Subject: Plant
- Issue Published:
The complete nucleotide sequence of the large (L) genome segment of tomato spotted wilt virus (TSWV) has been determined. The RNA is 8897 nucleotides long and contains complementary 3′ and 5′ ends, comprising 62 nucleotides at the 5′ end and 66 nucleotides at the 3′ end. The RNA is of negative polarity, with one large open reading frame (ORF) located on the viral complementary strand. This ORF corresponds to a primary translation product of 2875 amino acids in length, with a predicted M r of 331500. Comparison with the polymerase proteins of other negative-strand viruses indicates that this protein most likely represents the viral polymerase. The genetic organization of TSWV L RNA is similar to that of the L RNA segments of Bunyamwera and Hantaan viruses, animal-infecting representatives of the Bunyaviridae.
© Society for General Microbiology 1991 | Published by the Microbiology Society
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