RT Journal Article SR Electronic(1) A1 Ohizumi, Yoshifumi A1 Suzuki, Hiroshi A1 Matsumoto, Yoh-Ichi A1 Masuho, Yasuhiko A1 Numazaki, YoshioYR 1992 T1 Neutralizing Mechanisms of Two Human Monoclonal Antibodies Against Human Cytomegalovirus Glycoprotein 130/55 JF Journal of General Virology, VO 73 IS 10 SP 2705 OP 2707 DO https://doi.org/10.1099/0022-1317-73-10-2705 PB Microbiology Society, SN 1465-2099, AB The neutralization of human cytomegalovirus (HCMV) after adsorption to the cell surface at 4 °C was studied using two neutralizing monoclonal antibodies (C-23 and C-41) recognizing glycoprotein 130/55. HCMV adsorbed to cells was neutralized by C-23 (complement-independent), but not by C-41 (complement-dependent). Furthermore, the virus remained sensitive to C-23 for 120 min after shifting up from 4 °C to 37 °C, suggesting that C-23 might block an early stage of virus penetration into cells, and also that transition from virus attachment to virus penetration might be quite slow. The cell-to-cell infection of HCMV was also blocked only by C-23, and not by C-41. On the basis of the results presented here, we suggest that C-41 blocks the attachment of virus to the cell surface whereas C-23 prevents the penetration of virus into the cell., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-10-2705