1887

Journal of General Virology header logo

Volume 73, Issue 12

Molecular basis of hepatitis B virus serotype variations within the four major subtypes Free

Abstract

Amino acid residues 101 to 180 of hepatitis B surface antigen (HBsAg) were predicted by sequencing the corresponding part of the S gene of hepatitis B virus (HBV) DNA in 46 HBsAg-positive sera, which had been subtyped by immunodiffusion with respect to to and . The sequences of the nine different HBV serotypes defined by these specificities were found to be homogeneous proving that they represent consistent variations of HBV at the genomic level. Residue 127 was found to be important as were Pro, Thr and Leu for and , respectively. Five residues were found to differ between and . These were at positions 134 (Phe instead of Tyr), 143 (Thr instead of Ser), 159 (Ala instead of Gly), 161 (Tyr instead of Phe) and 168 (Val instead of Ala). However, all these residues were shared by and , implying that Arg was also important for expression. All genomes expressing , apart from one strain, had an Ile, which might explain the pseudo-allelism of to in relation to , since this substitution might influence the epitope. There were two regions where and differed from all the subtypes. These were located at residues 158 and 159, and at residues 177 and 178, where both the subtypes had amino acid substitutions in adjacent positions. The mapping of the epitopes defining these antigenic specificities will help to link information on the world-wide distribution of HBsAg subtypes to future molecular epidemiology with regard to HBV.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-12-3141
1992-12-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/12/JV0730123141.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-12-3141&mimeType=html&fmt=ahah

References

  1. Ashton-Rickardt P. G., Murray K. 1989; Mutants of the hepatitis B virus surface antigen that define some antigenically essential residues in the immunodominant a region. Journal of Medical Virology 29:196–203
     [Google Scholar]
  2. Bancroft W. H., Mundon F. K., Russell P. K. 1972; Detection of additional antigenic determinants of hepatitis B antigen. Journal of Immunology 109:842–848
     [Google Scholar]
  3. Ben-Porath E., Wands J. R., Marciniak R. A., Wong M. A., Hornstein L., Ryder R., Canias M., Lingao A., Isselbacher K. J. 1985; Structural analysis of hepatitis B surface antigen by monoclonal antibodies. Journal of Clinical Investigation 76:1338–1347
     [Google Scholar]
  4. Bhatanagar P. K., Papas E., Blum H. E., Milich D. R., Nitecki D., Karels M. J., Vyas G. N. 1982; Immune response to synthetic peptide analogues of hepatitis B surface antigen specific for the a determinant. Proceedings of the National Academy of Sciences, U.S.A. 79:4400–4404
     [Google Scholar]
  5. Brown S. E., FIoward C. R., Zuckerman A. J., Steward M. W. 1984; Affinity of antibody responses in man to hepatitis B vaccine determined with synthetic peptides. Lancet ii:184–187
     [Google Scholar]
  6. Couroucé A. M., Holland P. V., Muller J. Y., Soulier J. P. 1976; HBs antigen subtypes. Bibliotheca Haematologica 42:1
     [Google Scholar]
  7. Couroucé-Pauty A. M., Soulier J. P. 1974; Further data on HBs antigen subtypes – geographical distribution. Vox Sanguinis 27:533–549
     [Google Scholar]
  8. Couroucé-Pauty A. M., Lemaire J. M., Roux J. F. 1978; New hepatitis B surface antigen subtypes inside the ad category. Vox Sanguinis 35:304–308
     [Google Scholar]
  9. Couroucé-Pauty A. M., Plancon A., Soulier J. P. 1983; Distribution of HBsAg subtypes in the world. Vox Sanguinis 44:197–211
     [Google Scholar]
  10. Dreesman G. R., Sanchez Y., Ionescu-Matiu I., Sparrow J. T., Six H. R., Peterson D. L., Hollinger B., Melnick J. L. 1982; Antibody to hepatitis B surface antigen after a single inoculation of uncoupled synthetic HBsAg peptides. Nature, London 295:158–160
     [Google Scholar]
  11. Gerin J. L., Alexander H., Shih J. W. K., Purcell R. H., Dapolito G., Engle R., Green N., Sutcliffe G., Shinnick T. M., Lerner R. A. 1983; Chemically synthesized peptides of hepatitis B surface antigen duplicate the d/y specificities and induce subtype specific antibodies in chipanzees. Proceedings of the National Academy of Sciences, U.S.A. 80:2365–2369
     [Google Scholar]
  12. Gerlich W. H., Heermann K.-H. 1991; Functions of hepatitis B virus proteins and virus assembly. In Viral Hepatitis and Liver Disease pp 121–134 Edited by Hollinger B., Lemon S., Margolis B. New York: Alan R. Liss;
     [Google Scholar]
  13. Howard C. R., Stirk H., Brown S. E., Steward M. W. 1988; Towards the development of synthetic hepatitis B vaccines. In Viral Hepatitis and Liver Disease pp 1094–1101 Edited by Zuckerman A. J. New York: Alan R. Liss;
     [Google Scholar]
  14. Imai M., Gotoh A., Nishioka K., Kurashina S., Miyakawa Y., Mayumi M. 1974; Antigenicity of reduced and alkylated Australia antigen. Journal of Immunology 112:416–449
     [Google Scholar]
  15. Larzul D., Guige F., Sninsky J. J., Mack D. H., Brechot C., Guedson J. L. 1988; Detection of hepatitis B virus sequences in serum by using in vitro enzymatic amplification. Journal of Virological Methods 20:227–237
     [Google Scholar]
  16. Le Bouvier G. L. 1971; The heterogeneity of Australia antigen. Journal of Infectious Diseases 123:671–675
     [Google Scholar]
  17. Lerner R. A., Green N., Alexander H., Liu F.-T., Sutcliffe J. G., Shinnick T. M. 1981; Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles. Proceedings of the National Academy of Sciences, U.S.A. 78:3403–3407
     [Google Scholar]
  18. Magnius L. O., Kaplan L., Vyas G. N., Perkins H. A. 1975; A new virus specified determinant of hepatitis B surface antigen. Acta Pathologica et Microbiologica Scandinavica 83B:295–297
     [Google Scholar]
  19. Norder H., Hammas B., Magnius L. O. 1990; Typing of hepatitis B virus genomes by a simplified polymerase chain reaction. Journal of Medical Virology 31:215–221
     [Google Scholar]
  20. Norder H., Hammas B., Löfdahl S., Couroucé A.-M., Magnius L. O. 1992; Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. Journal of General Virology 73:1201–1208
     [Google Scholar]
  21. Ohnuma H., Takai E., Machida A., Tsuda F., Okamoto H., Tanaka T., Naito M., Munekata E., Miki K., Miyakawa Y., Mayumi M. 1990; Synthetic oligopeptides bearing a common or subtypic determinant of hepatitis B surface antigen. Journal of Immunology 145:2265–2271
     [Google Scholar]
  22. Okamoto H., Imai M., Tsuda F., Tanaka T., Miyakawa Y., Mayumi M. 1987; Point mutation in the S-gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr . Journal of Virology 61:3030–3034
     [Google Scholar]
  23. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. Journal of General Virology 69:2575–2583
     [Google Scholar]
  24. Sastrosoewignjo R., Sandjaja B., Okamoto H. 1991; Molecular epidemiology of hepatitis B virus in Indonesia. Journal of Gastro-enterology and Hepatology 6:491–498
     [Google Scholar]
  25. Shorey J. 1976; A new hepatitis B surface antigen. Journal of Infectious Diseases 133:1–6
     [Google Scholar]
  26. Soulier J. P., Couroucé-Pauty A. M. 1973; New determinants of hepatitis B antigen (Au or Hb antigen). Vox Sanguinis 25:212–234
     [Google Scholar]
  27. Swenson P. D., Riess J. T., Krueger L. E. 1991; Determination of HBsAg subtypes in different high risk populations using monoclonal antibodies. Journal of Virological Methods 33:27–28
     [Google Scholar]
  28. Valenzuela P., Quiroga M., Zaldivar J., Gray P., Rutter W. J. 1980; The nucleotide sequence of the hepatitis B viral genome and the identification of the major viral genes. In ICN–UCLA Symposia on Animal Virus Genetics pp 57–70 Edited by Fields B., Jaenisch R., Fox C. New York: Academic Press;
     [Google Scholar]
  29. Wands J. R., Wong M. A., Shorey J., Brown R. D., Marciniak R. A., Isselbacher K. J. 1984; Hepatitis B viral antigenic structure: signature analysis by monoclonal radioimmunoassays. Proceedings of the National Academy of Sciences, U.S.A. 81:2237–2241
     [Google Scholar]
  30. Waters J. A., Brown S. E., Steward M. W., Howard C. R., Thomas H. C. 1992; Analysis of the antigenic epitopes of hepatitis B surface antigen involved in the induction of a protective antibody response. Virus Research 22:1–12
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-12-3141
Loading
Molecular basis of hepatitis B virus serotype variations within the four major subtypes
J. Gen. Virol. 73, 3141 (1992); https://doi.org/10.1099/0022-1317-73-12-3141
/content/journal/jgv/10.1099/0022-1317-73-12-3141
/content/journal/jgv/10.1099/0022-1317-73-12-3141
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error