RT Journal Article SR Electronic(1) A1 Sunil-Chandra, N. P. A1 Efstathiou, S. A1 Nash, A. A.YR 1992 T1 Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo JF Journal of General Virology, VO 73 IS 12 SP 3275 OP 3279 DO https://doi.org/10.1099/0022-1317-73-12-3275 PB Microbiology Society, SN 1465-2099, AB Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein—Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-12-3275