1887

Abstract

Cytomegaloviruses generally display a host range restricted to differentiated cell types from the species they infect. For human cytomegalovirus (HCMV) this has meant that with few exceptions tissue culture systems have relied on the use of primary foreskin fibroblast (HF) cells or primary human embryonic lung cells to study gene expression and virus replication functions. We have observed that primary skin fibroblast (CF) cells derived from the chimpanzee () support the replication of a laboratory strain (Towne) of HCMV. The kinetics of gene expression of the Towne strain grown in CF or HF cells appeared to be equivalent. Titres of progeny virions grown in CF cells appeared to be reduced 10-fold relative to those of virus grown in HF cells. In contrast, replication of the Towne virus was not supported by growth in WES cells (ATCC no. CRL 1609), a chimpanzee skin fibroblast cell line transformed by an adenovirus 12-simian virus 40 hybrid. This study shows that HCMV is less parochial in its host range than previously thought.

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1992-12-01
2024-04-20
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References

  1. Cao L., Faha B., Dembski M., Tsai L.-H., Harlow E., Dyson N. 1992; Independent binding of the retinoblastoma protein and pl07 to the transcription factor E2F. Nature, London 355:176–179
    [Google Scholar]
  2. De Grouchy J. 1987; Chromosome phylogenies of man, great apes, and old world monkeys. Genetica 73:37–52
    [Google Scholar]
  3. Faha B., Ewen M. E., Tsai L.-H., Livingston D. M., Harlow E. 1992; Interaction between human cyclin A and adenovirus E1A-associated pl07 protein. Science 255:87–90
    [Google Scholar]
  4. Gonczol E., Andrews P. W., Plotkin S. A. 1984; Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells. Science 224:159–161
    [Google Scholar]
  5. Ho M. 1991 Cytomegalovirus: Biology and Infection New York & London: Plenum Press;
    [Google Scholar]
  6. Kari B., Radeke R., Gehrz R. 1992; Processing of human cytomegalovirus envelope glycoproteins in and egress of cytomegalovirus from human astrocytoma cells. Journal of General Virology 73:253–260
    [Google Scholar]
  7. LaFemina R. L., Hayward G. S. 1988; Differences in cell type-specific blocks to immediate early gene expression and DNA replication of human, simian and murine cytomegalovirus. Journal of General Virology 69:355–374
    [Google Scholar]
  8. Liu B., Stinski M. F. 1992; Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cfi-acting elements. Journal of Virology 66:4434–4444
    [Google Scholar]
  9. Pachl C., Burke R. L., Stuve L. L., Sanchez-Pescador L., Van Nest G., Masiarz F., Dina D. 1987; Expression of cell-associated and secreted forms of herpes simplex virus type 1 glycoprotein gB in mammalian cells. Journal of Virology 61:315–325
    [Google Scholar]
  10. Rasmussen L., Mullenax J., Nelson R., Merigan T. C. 1985; Viral polypeptides detected by a complement-dependent neutralizing murine monoclonal antibody to human cytomegalovirus. Journal of Virology 55:274–280
    [Google Scholar]
  11. Rhim J. S., Trimmer R., Arnstein P., Huebner R. J. 1981; Neoplastic transformation of chimpanzee cells induced by adenovirus type 12-simian virus 40 hybrid virus. Proceedings of the National Academy of Sciences, U.S.A. 78:313–317
    [Google Scholar]
  12. Rice G. P. A., Schrier R. D., Oldstone M. B. A. 1984; Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proceedings of the National Academy of Sciences, U.S.A. 81:6134–6138
    [Google Scholar]
  13. Smith J. D. 1986; Human cytomegalovirus: demonstration of permissive epithelial cells and nonpermissive fibroblastic cells in a survey of human cell lines. Journal of Virology 60:583–588
    [Google Scholar]
  14. Spaete R. R., Mocarski E. S. 1985; Regulation of cytomegalovirus gene expression: α and β promoters are trans activated by viral functions in permissive human fibroblasts. Journal of Virology 56:135–143
    [Google Scholar]
  15. Spaete R. R., Thayer R. M., Probert W. S., Masiarz F. R., Chamberlain S. H., Rasmussen L., Merigan T. C., Pachl C. 1988; Human cytomegalovirus strain Towne glycoprotein B is processed by proteolytic cleavage. Virology 167:207–225
    [Google Scholar]
  16. Stinski M. F., Roehr T. J. 1985; Activation of the major immediate early gene of human cytomegalovirus by eu-acting elements in the promoter-regulatory sequence and by virus-specific trans-acting components. Journal of Virology 55:431–441
    [Google Scholar]
  17. Swinkels B. W., Geelen J. L. M. C., Wertheim-van Dillen P., van Es A. A., van der Noordaa J. 1984; Initial characterization of four cytomegalovirus strains isolated from chimpanzees. Archives of Virology 82:125–128
    [Google Scholar]
  18. Weiner D., Gibson W. 1981; Identification of a primate cytomegalovirus group-common protein antigen. Virology 115:182–191
    [Google Scholar]
  19. Wroblewska Z., Wellish M. C., Wolinsky J. S., Gilden D. 1981; Comparison of human cytomegalovirus growth in MRC-5 human fibroblasts, brain, and choroid plexus cells in vitro . Journal of Medical Virology 8245–256
    [Google Scholar]
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