Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues
Welch, Hazel M. and Bridges, C. Gordon and Lyon, Allyson M. and Griffiths, Lyn and Edington, Neil,, 73, 261-268 (1992),
doi = https://doi.org/10.1099/0022-1317-73-2-261,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The polymerase chain reaction (PCR) and co-cultivation were used to identify the lymphoreticular system as the site of latency of equid herpesvirus I (EHV-1). Primers for PCR were designed from aligned nucleotide sequences of the glycoprotein gB genes to amplify the same region of both the EHV-1 and EHV-4 genomes. Subsequent restriction digests using specific enzymes distinguished the amplified fragments of the EHV-1 genome from those of the EHV-4 genome. Ten weeks following an experimental infection of five ponies with EHV-1, latent virus was detected by PCR and recovered by co-cultivation, predominantly from lymphoid tissues draining the respiratory tract. Significantly, latent EHV-1 also persisted in peripheral blood leukocytes (PBL). Latent EHV-4, presumably from a preceding natural infection, was also detected in some tissues, including PBL, from all animals. Of additional interest was the recovery of EHV-1 and -4 only in the presence of the ubiquitous EHV-2.,
language=,
type=