1887

Abstract

The viral and host factors involved in herpes simplex virus (HSV) recombination are little understood. To identify features of the process, recombination in HSV-1 and HSV-2 has been studied by analysing the segregation of unselected markers in the form of restriction endonuclease (RE) sites. By confining parental interactions to only one strain of virus of each serotype, restrictions imposed by non-homology are overcome and differential growth phenotypes can be discounted. The analysis of unselected and selected recombinants using RE sites in conjunction with temperature-sensitive mutations is consistent with (i) HSV being highly recombinogenic, (ii) parental and progeny molecules taking part in the process, (iii) the four genomic isomers participating in recombination, (iv) genome alignment being part of the recombination process and (v) cellular factors in conjunction with genome homology influencing the efficiency of recombination.

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1992-02-01
2024-04-20
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References

  1. Brown S. M., Ritchie D. A., Subak-Sharpe J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature–sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
    [Google Scholar]
  2. Brown S. M., Harland J., Subak–Sharpe J. H. 1984; Isolation of restriction endonuclease site deletion mutants of herpes simplex virus. Journal of General Virology 65:1053–1068
    [Google Scholar]
  3. Cook S. D., Brown S. M. 1987; Herpes simplex virus type 1 latency in rabbit corneal cells in vitro: reactivation and recombination following intratypic superinfection of long term cultures. Journal of General Virology 68:813–824
    [Google Scholar]
  4. Cortini R., Wilkie N. M. 1978; Physical maps for HSV type 2 DNA with five restriction endonucleases. Journal of General Virology 39:259–280
    [Google Scholar]
  5. Harland J., Brown S. M. 1988; Generation of a herpes simplex virus type 2 variant devoid of Xba I sites: removal of the 0.91 map coordinate site results in impaired synthesis of glycoprotein G–2. Journal of General Virology 69:113–124
    [Google Scholar]
  6. Honess R. W., Buchan A., Halliburton I. W., Watson D. H. 1980; Recombination and linkage between structural and regulatory genes of herpes simplex virus type 1: study of the functional organisation of the genome. Journal of Virology 34:716–742
    [Google Scholar]
  7. Lonsdale D. H. 1979; A rapid technique for distinguishing herpes simplex virus type 1 from type 2 by restriction enzyme technology. Lancet i:849–852
    [Google Scholar]
  8. MacLean A. R., Brown S. M. 1987a; Generation of a herpes simplex virus type 1 variant devoid of Xba I sites. Journal of General Virology 68:1165–1171
    [Google Scholar]
  9. MacLean A. R., Brown S. M. 1987b; Deletion and duplication variants around the long repeats of herpes simplex virus type 1 strain 17. Journal of General Virology 68:3019–3031
    [Google Scholar]
  10. Preston V. G., Coates J. A. V., Rixon F. J. 1983; Identification and characterisation of a herpes simplex virus gene product required for encapsidation of virus DNA. Journal of Virology 45:1056–1064
    [Google Scholar]
  11. Schaffer P. A., Telethia M. J., Benyesh-Melnick M. 1974; Recombination between temperature sensitive mutants of herpes simplex virus type 1. Virology 58:219–228
    [Google Scholar]
  12. Stow N. D., Subak-Sharpe J. H., Wilkie N. M. 1978; Physical mapping of HSV1 mutations by marker rescue. Journal of Virology 28:182–192
    [Google Scholar]
  13. Subak–Sharpe J. H. 1969; Herpes simplex virus recombination. In Proceedings of the First international Congress for Virology 1968 p 252 Edited by Melnick J. L. Helsinki: S. Karger;
    [Google Scholar]
  14. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
  15. Timbury M.C., Calder L. 1976; Temperature–sensitive mutants of herpes simplex virus type 2: a provisional linkage map based on recombinational analysis. Journal of General Virology 30:179–186
    [Google Scholar]
  16. Umene K. 1985; Intermolecular recombination of the herpes simplex virus type 1 genome analysed using two strains differing in restriction enzyme cleavage sites. Journal of General Virology 66:2659–2670
    [Google Scholar]
  17. Wildy P. 1955; Recombination with herpes simplex virus. Journal of General Microbiology 13346–363
    [Google Scholar]
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