1887

Abstract

Consensus cis-acting DNA sequences upstream of the immediate early (IE) gene of equid herpesvirus type 1 (EHV-1, strain Ab4) were identified. One copy of the conserved motif TAATGARATTC, which is the binding site for the host cellular factor Oct-1 and herpes simplex virus type 1 (HSV-1) virion protein, VmW65, complex, was identified at positions -630 to -620. Using transient transfections and chloramphenicol acetyltransferase assays the IE promoter of EHV-1 was shown to be trans-activated by VmW65 within the region -685 to +73. Ultraviolet light-inactivated EHV-1 was able to stimulate the expression of the IE gene of EHV-1 as well as HSV-1, indicating that EHV-1 possesses a protein equivalent to VmW65. The ubiquitous equid herpesvirus type 2 (EHV-2), which is not known to be a primary pathogen, was also able to trans-activate the EHV-1 and HSV-1 IE genes. Further work is being performed in order to identify the nature of the EHV-1 and EHV-2 trans-activating proteins.

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1992-03-01
2024-03-28
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References

  1. Ace C. I., McKee T. A., Ryan J. M., Cameron J. M., Preston C. M. 1989; Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate–early gene expression. Journal of Virology 63:2260–2269
    [Google Scholar]
  2. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus–1 infections. Progress in Veterinary Microbiology and Immunology 2:78–144
    [Google Scholar]
  3. Allen G. P., Turtinen L. W. 1982; Assessment of the base sequence homology between the two subtypes of equine herpesvirus 1. Journal of Virology 44:249–255
    [Google Scholar]
  4. Baumann R. P., Yalamanchili V. R. R., O’Callaghan D. J. 1989; Functional mapping and DNA sequence of an equine herpesvirus 1 origin of replication. Journal of Virology 63:1275–1283
    [Google Scholar]
  5. Blakeslee J. R. Jr, Olsen R. G., McAllister E. S., Fassbender J., Dennis R. 1975; Evidence of respiratory tract infection induced by equine herpesvirus type 2, in the horse. Canadian Journal of Microbiology 21:1940–1946
    [Google Scholar]
  6. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  7. Campbell M. E. M., Preston C. M. 1987; DNA sequences which regulate the expression of the pseudorabies virus major immediate early gene. Virology 157:307–316
    [Google Scholar]
  8. Campbell M. E. M., Palfreyman J. W., Preston C. M. 1984; Identification of herpes simplex virus DNA sequences which encode a transacting polypeptide responsible for stimulation of immediate early transcription. Journal of Molecular Biology 180:1–19
    [Google Scholar]
  9. Clements J. B., Watson R. J., Wilkie N. M. 1977; Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome. Cell 12:275–285
    [Google Scholar]
  10. Colacino J. M., Flowers C. C., Menna J., O’Callaghan D. J., Staczek J. 1989; Physical structure and molecular cloning of equine cytomegalovirus DNA. Virology 173:566–580
    [Google Scholar]
  11. Cordingley M. G., Campbell M. E. M., Preston C. M. 1983; Functional analysis of a herpes simplex virus type 1 promoter: identification of far–upstream regulatory sequences. Nucleic Acids Research 8:2347–2365
    [Google Scholar]
  12. Cousens D. J., Greaves R., Goding C. R., O’Hare P. 1989; The C–terminal 79 amino acids of the herpes simplex virus regulatory protein, VmW65, efficiently activate transcription in yeast and mammalian cells in chimeric DNA–binding proteins. EMBO Journal 8:2337–2342
    [Google Scholar]
  13. Edington N. 1991; Latency of equine herpesviruses. VIth International Conference of Equine Infectious Diseases Cambridge; U.K.: (in press)
    [Google Scholar]
  14. Edington N., Bridges C. G., Huckle A. 1985; Experimental reactivation of equid herpesvirus 1 (EHV–1) following administration of corticosteroids. Equine Veterinary Journal 17:369372
    [Google Scholar]
  15. Everett R. D. 1987; The regulation of transcription of viral and cellular genes by herpesvirus immediateearly gene products. Anticancer Research 7:589–604
    [Google Scholar]
  16. Fu Z. F., Robinson A. J., Horner G. W., Dickinson L. G., Grimett J. B., Marshall R. B. 1986; Respiratory disease in foals and the epizootiology of equine herpesvirus type 2 infection. New Zealand Veterinary Journal 34:152
    [Google Scholar]
  17. Gaffney D. F., McLauchlan J., Whitton J. L., Clements J. B. 1985; A modular system for the assay of transcription regulatory signals: the sequence TAATGARAT is required for herpes simplex virus immediate early gene activation. Nucleic Acids Research 13:7847–7863
    [Google Scholar]
  18. Gorman C. M., Moffatt L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  19. Gray W. L., Baumann R. P., Robertson A. T., Caughman G. B., O’Callaghan D. J., Staczek J. 1987; Regulation of EHV–1 type 1 gene expression: characterization of immediate early, early, and late transcription. Virology 158:79–87
    [Google Scholar]
  20. Grundy F. J., Baumann R. P., O’Callaghan D. J. 1989; DNA sequence and comparative analyses of the equine herpesvirus type 1 immediate early gene. Virology 172:223–236
    [Google Scholar]
  21. Harty R. N., Colle C. F., Grundy F. J., O’Callaghan D. J. 1989; Mapping the termini and intron of the spliced immediate-early transcript of equine herpesvirus 1. Journal of Virology 63:5101–5110
    [Google Scholar]
  22. Honess R. W., Roizman B. 1974; Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
    [Google Scholar]
  23. Jones K. A., Yamamoto K. R. 1985; Two distinct transcription factors bind to the HSV thymidine kinase promoters in vitro . Cell 42:559–572
    [Google Scholar]
  24. Kemeny L., Pearson J. E. 1970; Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus. Canadian Journal of Comparative Medicine 34:59–65
    [Google Scholar]
  25. Kozmik Z., Arnold L., Paces V. 1991; Multiple sets of adjacent µE1 and Oct-1 binding sites upstream of the pseudorabies virus immediate-early gene promoter. Virology 182:239–249
    [Google Scholar]
  26. McKee T. A., Disney G. H., Everett R. D., Preston C. M. 1990; Control of expression of the varicella-zoster virus major immediate early gene. Journal of General Virology 71:897–906
    [Google Scholar]
  27. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Marsden H. S., Campbell M. E. M., Haarr L., Frame M. C., Parris D. S., Murphy M., Hope R. G., Muller M. G., Preston C. M. 1987; The 65, 000 Mr DNA–binding and virion trans–inducing proteins of herpes simplex virus type 1. Journal of Virology 61:2428–2437
    [Google Scholar]
  29. Notarianni E. L., Preston C. M. 1982; Activation of cellular stress protein genes by herpes simplex virus temperature–sensitive mutants which overproduce immediate early polypeptides. Virology 123:113–122
    [Google Scholar]
  30. O’Hare P. C., Goding C. R., Haigh A. 1988; Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer-binding factor mediates specific induction of virus immediate-early gene expression. EMBO Journal 7:4231–4238
    [Google Scholar]
  31. Patel J. R., Edington N. 1983; The pathogenicity in mice of respiratory, abortion and paresis isolates of equine herpesvirus-1. Veterinary Microbiology 8:301–305
    [Google Scholar]
  32. Perbal B. V. 1988 A Practical Guide to Molecular Cloning New York: John Wiley;
    [Google Scholar]
  33. Preston C. M., Cordingley M. G., Stow N. D. 1984; Analysis of DNA sequences which regulate the transcripion of a herpes simplex virus immediate early gene. Journal of Virology 50:708–716
    [Google Scholar]
  34. Preston C. M., Frame M. C., Campbell M. E. M. 1988; A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence. Cell 52:425–434
    [Google Scholar]
  35. Sadowski I., Ma J., Triezenberg S., Ptashne M. 1988; GAL4–VP16 is an unusually potent transcriptional activator. Nature, London 335:563–564
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain–terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  37. Staczek J., Atherton S. S., O’Callaghan D. J. 1983; Genetic relatedness of the genomes of equine herpesvirus types 1, 2 and 3. Journal of Virology 45:855–858
    [Google Scholar]
  38. Studdert M. J. 1974; Comparative aspects of equine herpesviruses. Cornell Veterinarian 64:94–122
    [Google Scholar]
  39. Triezenberg S. J., LaMarco K. L., McKnight S. L. 1988; Evidence of DNA: protein interactions that mediate HSV–1 immediate-early gene activation by VP16. Genes and Development 2:730–742
    [Google Scholar]
  40. Welch H. M., Bridges C. G., Lyon A. M., Griffiths L., Edington N. 1992; Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and cocultivation from lymphoid tissues. Journal of General Virology 73:261–268
    [Google Scholar]
  41. Wilbur W. J., Lipman D. J. 1983; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences, U.S.A 80:726–730
    [Google Scholar]
  42. Yalamanchili R. R., O’Callaghan D. J. 1990; Organization and function of the Oris sequence in the genome of EHV–1 DI particles. Virology 179867–870
    [Google Scholar]
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