Extraordinarily low density of hepatitis C virus estimated by sucrose density gradient centrifugation and the polymerase chain reaction
Miyamoto, Hideaki and Okamoto, Hiroaki and Sato, Koei and Tanaka, Takeshi and Mishiro, Shunji,, 73, 715-718 (1992),
doi = https://doi.org/10.1099/0022-1317-73-3-715,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The genomic RNA of hepatitis C virus (HCV) in the plasma of volunteer blood donors was detected by using the polymerase chain reaction in a fraction of density 1.08 g/ml from sucrose density gradient equilibrium centrifugation. When the fraction was treated with the detergent NP40 and recentrifuged in sucrose, the HCV RNA banded at 1.25 g/ml. Assuming that NP40 removed a lipid-rich surface coat from HCV, the 1.08 g/ml and 1.25 g/ml HCV RNA may correspond to intact HCV virions and nucleocapsids, respectively. The extraordinarily low density of the virion is unusual in comparison to the density of classified viruses.,
language=,
type=