1887

Abstract

Immunodominant, conserved and type-restricted external epitopes of bovine papillomavirus (BPV) major (L1) capsid protein have been identified using BPV particles and synthetic peptides. Antisera to disrupted BPV-1 recognized BPV-1 and BPV-2 particles in immune electron microscopy (IEM) studies and inhibited BPV-2-induced focus formation of NIH/3T3 cells. Thus BPV-1/BPV-2 cross-reactive epitopes occur on the surface of virions. The L1 protein appeared to be immunodominant as the antisera reacted with three dominant BPV-1/BPV-2 conserved B cell epitopes (amino acids 111 to 125, 131 to 145 and 191 to 205) in Pepscan assays of BPV-1 L1, whereas no common epitopes and less frequent antibody binding to peptides were detected in Pepscans of the L2 protein of BPV-1. Four discrete variable regions were identified in the sequences of L1 proteins of BPV-1 and BPV-2. Antisera against synthetic peptides corresponding to three of the four variable regions (amino acids 42 to 56, 435 to 449 and 485 to 499) of BPV-2 L1 caused clumping of BPV-2, but not of BPV-1, particles as examined by IEM, and antisera to one peptide (amino acids 485 to 499) inhibited BPV-2-induced focus formation of NIH/3T3 cells. These data suggest that these regions are type-specific BPV-2 L1 epitopes and that they occur on the virion surface. Although conformation-dependent epitopes remain to be identified on papillomaviruses, the linear epitopes identified in this study may be worthy of further study as constituents of experimental prophylactic vaccines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-12-2669
1993-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/12/JV0740122669.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-12-2669&mimeType=html&fmt=ahah

References

  1. Banks L., Matlashewski G., Pim D., Churcher M., Roberts C., Crawford L. 1987; Expression of human papillomavirus type 6 and 16 capsid proteins in bacteria and their antigenic characterization. Journal of General Virology 68:3081–3089
    [Google Scholar]
  2. Burke K. L., Evans D. J., Jenkins O., Meredith J., D’Souza E. D. A., Almond J. W. 1989; A cassette vector for the construction of antigen chimaeras of poliovirus. Journal of General Virology 70:2475–2479
    [Google Scholar]
  3. Cason J., Best J. M. 1991; Antibody responses to human papillomavirus type-16 infections. Reviews in Medical Virology 1:201–209
    [Google Scholar]
  4. Cason J., Patel D., Naylor J., Lunney D., Shepherd P. S., Best J. M., McCance D. J. 1989; Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes. Journal of General Virology 70:2973–2987
    [Google Scholar]
  5. Cason J., Kambo P. K., Best J. M., McCance D. J. 1992; Detection of antibodies to a linear epitope on the major capsid protein of human papillomavirus type-16 in sera from patients with cervical intraepithelial neoplasia and children. International Journal of Cancer 50:349–355
    [Google Scholar]
  6. Cason J., Kahn S. A., Best J. M. 1993; Towards vaccines against human papillomavirus type 16 genital infections. Vaccine 11:603–611
    [Google Scholar]
  7. Chen E. Y., Howley P. M., Levinson A. D., Seeburg P. H. 1982; The primary structure and genetic organization of the bovine papillomavirus type-1 genome. Nature, London 299:529–534
    [Google Scholar]
  8. Christensen N. D., Kreider J. W., Kan N. C., DiAngelo S. L. 1991; The open reading frame of L2 of cottontail rabbit papillomavirus contains antibody-inducing neutralizing epitopes. Virology 181:572–579
    [Google Scholar]
  9. Cole S. T., Danos O. 1987; Nucleotide sequence and comparative analysis of human papillomavirus type-18 genome: phylogeny of papillomaviruses and repeated structure of E6 and E7 gene products. Journal of Molecular Biology 193:599–608
    [Google Scholar]
  10. Dillner J., Dillner L., Utter G., Eklund C., Rotola A., Costa S., DiLuca D. 1990; Mapping of linear epitopes of human papillomavirus type 16: the L1 and L2 open reading frames. International Journal of Cancer 45:529–535
    [Google Scholar]
  11. Dillner L., Heino P., Moreno-Lopez J., Dillner J. 1991; Antigenic and immunogenic epitopes shared by human papillomavirus type 16 and bovine, canine and avian papillomaviruses. Journal of Virology 65:6862–6870
    [Google Scholar]
  12. Doolittle R. F. 1986 Of URFs and ORFs: A Primer on How to Analyse Derived Amino Acid Sequences California: University Science Books;
    [Google Scholar]
  13. Geysen H. M., Rodda S. J., Mason T. J., Tribbick G., Schoofs P. G. 1987; Strategies for epitope analysis using peptide synthesis. Journal of Immunological Methods 102:259–274
    [Google Scholar]
  14. Gissmann L., Schwartz E. 1986; Persistence and expression of human papillomavirus DNA in genital cancer. In Papillomaviruses, Ciba Foundation Symposium 120 pp 190–207 Chichester: John Wiley;
    [Google Scholar]
  15. Jarrett W. H. F., O’Neil B. W., Gaukroger J. M., Laird H. M., Smith K. T., Campo M. S. 1990; Studies on vaccination against papillomaviruses: a comparison of purified virus, tumor extract and transformed cells in prophylactic vaccination. Veterinary Record 126:449–452
    [Google Scholar]
  16. Jenison S. A., Yu X. P., Valentine J. M., Galloway D. A. 1989; Human antibodies react with an epitope of human papillomavirus type-6b L1 open reading frame which is distinct from the type-common epitope. Journal of Virology 63:809–818
    [Google Scholar]
  17. Jenison S. A., Yu X. P., Valentine J. M., Koutsky L. A., Christiansen A. E., Beckmann A. M., Galloway D. A. 1990; Evidence of prevalent genital-type human papillomavirus infections in adults and children. Journal of Infectious Diseases 162:60–69
    [Google Scholar]
  18. Jenison S. A., Yu X. P., Valentine J. M., Galloway D. A. 1991; Characterization of human antibody-reactive epitopes encoded by human papillomavirus types 16 and 18. Journal of Virology 65:1208–1218
    [Google Scholar]
  19. Jenkins O., Cason J., Burke K. L., Lunney D., Gillen A., Patel D., McCance D. J., Almond J. W. 1990; An antigenic chimera of poliovirus induces antibodies against human papillomavirus type 16. Journal of Virology 64:1201–1206
    [Google Scholar]
  20. Jensen A. B., Rosenthal J. R., Olson C., Pass F., Lancaster W. D., Shah K. 1980; Immunological relatedness of papillomaviruses from different species. Journal of the National Cancer Institute 64:495–500
    [Google Scholar]
  21. Lim P. S., Jensen A. B., Cowsert L. 1990; Distribution and specific identification of papillomavirus major capsid protein epitopes by immunocytochemistry and epitope scanning of synthetic peptides. Journal of Infectious Diseases 162:1263–1269
    [Google Scholar]
  22. Meyers C., Frattini M. G., Hudson J. B., Laimins L. A. 1992; Biosynthesis of human papillomavirus type 31b from a continuous cell line upon epithelial differentiation. Science 257:971–973
    [Google Scholar]
  23. Müller M., Gausepohl H., de Martynoff G., Frank R., Brasseur R., Gissmann L. 1990; Identification of seroreactive regions of the human papillomavirus type 16 proteins E4, E6, E7 and L1. Journal of General Virology 71:2709–2717
    [Google Scholar]
  24. Pilacinski W. P., Glassman D. L., Glassman K. F., Reed D. E., Lum M. A., Marshall R. F., Muscoplat C. C., Faras A. J. 1986; Immunization against bovine papillomavirus infection. In Papillomaviruses: Ciba Foundation Symposium 120 pp 136–156 Chichester: John Wiley;
    [Google Scholar]
  25. Potter H. L., Meinke W. J. 1985; Nucleotide sequence of bovine papillomavirus type 2 late region. Journal of General Virology 66:187–193
    [Google Scholar]
  26. Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W. G. 1985; Human papillomavirus type-16 DNA sequence. Virology 145:181–185
    [Google Scholar]
  27. Steger G., Olszewsky M., Stockfleth E., Pfister H. 1990; Prevalence of antibodies to human papillomavirus type 8 in human sera. Journal of Virology 64:4399–4406
    [Google Scholar]
  28. Sterling J., Stanley M., Gatward G., Minson T. 1990; Production of human papillomavirus type 16 virions in a keratinocyte cell line. Journal of Virology 64:6305–6307
    [Google Scholar]
  29. Strike D. G., Bonnez W., Rose R. C., Reichman R. C. 1989; Expression in Escherichia coli of seven DNA fragments comprising the complete L1 and L2 open reading frames of human papillomavirus type 6b and localization of the ‘common antigenic’ region. Journal of General Virology 70:543–555
    [Google Scholar]
  30. Sundberg J. P. 1987; Animal papillomaviruses. In Papillomaviruses and Human Disease pp 40–103 Edited by Syrjanen K., Gissmann L., Koss L. G. Berlin: Springer-Verlag;
    [Google Scholar]
  31. Yaegashi N., Jenison S. A., Valentine J. M., Dunn M., Taichman L. B., Baker D. A., Galloway D. A. 1991; Characterization of murine polyclonal antisera and monoclonal antibodies generated against intact and denatured human papillomavirus type 1 virions. Journal of Virology 65:1578–1583
    [Google Scholar]
  32. Yaegashi N., Jenison S. A., Batra M., Galloway D. A. 1992; Human antibodies recognize multiple distinct type-specific and cross-reactive regions of the minor capsid proteins of human papillomavirus types 6 and 11. Journal of Virology 66:2008–2019
    [Google Scholar]
  33. Zhou J., Sun X.-Y., Crawford L., Park D., Frazer I. H. 1992; Definition of linear antigenic regions of the HPV-16 L1 capsid protein using synthetic virion-like particles. Virology 189:592–599
    [Google Scholar]
  34. zur Hausen H., Schneider A. 1987; The role of papillomaviruses in human anogenital cancer. In The Papovaviridae vol 2 The Papillomaviruses, pp 245–263 Edited by Salzman N. P., Howley P. M. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-12-2669
Loading
/content/journal/jgv/10.1099/0022-1317-74-12-2669
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error