1887

Abstract

A herpesvirus of turkeys (HVT) recombinant containing a 3.9 kbp fragment of Marek's disease virus (MDV) DNA encoding MDV glycoprotein B (gB), stably integrated into the thymidine kinase (TK) gene of HVT, has been constructed. The replication of the recombinant in chick embryo fibroblasts (CEF) was comparable to that of wild-type HVT. The recombinant expressed authentic MDV gB and its processed forms (110K, 65K and 48K) in CEF as shown by immunoblotting using an MDV-specific anti-peptide serum. Northern blot analysis showed that MDV gB mRNA was transcribed from MDV promoter sequences flanking the MDV gB open reading frame and also from the HVT TK promoter. However, the level of replication of the recombinant appeared to be lower than wild-type HVT as shown by the titres of HVT antibodies, determined by ELISA. Pathogenicity tests showed that the recombinant was safe and did not cause microscopic or gross Marek's disease lesions or other abnormalities. The results suggest that HVT has potential as a vector for recombinant vaccines.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-3-371
1993-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/3/JV0740030371.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-3-371&mimeType=html&fmt=ahah

References

  1. Bayliss C. D., Peters D. W., Cook K. A., Reece R. L., Howes K., Binns M. M., Boursnell M. E. G. 1991; A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus. Archives of Virology 120:193–205
    [Google Scholar]
  2. Biggs P. M., Milne B. 1972 Biological properties of a number of Marek’s disease virus isolates. In Oncogenesis and Herpesviruses pp. 88–94 Edited by Biggs P. M., de The G., Payne L. N. Lyon: I ARC Scientific Publications;
    [Google Scholar]
  3. Boursnell M. E. G., Green P. F., Samson A. C. R., Campbell J. I. A., Deuter A., Peters R. W., Millar N. S., Emmerson P. T., Binns M. M. 1990; A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge by NDV. Virology 178:297–300
    [Google Scholar]
  4. Calnek B. W., Adldinger H. K., Kahn D. E. 1970; Feather follicle epithelium: a source of enveloped and infectious cell-free herpesvirus from Marek’s disease. Avian Diseases 14:219–233
    [Google Scholar]
  5. Cantello J. L., Anderson A. S., Francesconi A., Morgan R. W. 1991; Isolation of a Marek’s disease virus (MDV) recombinant containing the lacZ gene of Escherichia coli stably inserted within the MDV US2 gene. Journal of Virology 65:1584–1588
    [Google Scholar]
  6. Lee Y. S., Tanaka A., Lau R. Y., Nonoyama M., Rabin H. 1980; Comparative studies of herpesvirus papio (baboon herpesvirus) DNA and Epstein-Barr virus DNA. Journal of General Virology 51:245–253
    [Google Scholar]
  7. Lowe R. S., Keller P. M., Keech B. J., Davison A. L, Whang Y., Morgan A. J., Kieff E., Ellis R. W. 1987; Varicella-zoster virus as a live vector for the expression of foreign genes. Proceedings of the National Academy of Sciences, U.S.A. 84:3896–3900
    [Google Scholar]
  8. Mockett A., Southee D., Tomley F., Deuter A. 1987; Fowlpox virus: its structural proteins and immunogens and the detection of viral-specific antibodies by ELISA. Avian Pathology 16:493–504
    [Google Scholar]
  9. Ross L. J. N., Basarab O., Walker D. J., Whitby B. 1975; Serological relationship between a pathogenic strain of Marek’s disease virus, its attenuated derivative and herpesvirus of turkeys. Journal of General Virology 28:37–47
    [Google Scholar]
  10. Ross L. J. N., Sanderson M., Scott S. D., Binns M. M., Doel T., Milne B. 1989; Nucleotide sequence and characterization of the Marek’s disease virus homologue of glycoprotein B of herpes simplex virus. Journal of General Virology 70:1789–1804
    [Google Scholar]
  11. Ross N. L. J., DeLorbe W., Varmus H. E., Bishop M. J., Brahic M., Haase A. 1981; Persistence and expression of Marek’s disease virus DNA in tumour cells and peripheral nerves studied by in situ hybridization. Journal of General Virology 57:285–296
    [Google Scholar]
  12. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Schat K. A., Calnek B. W., Fabricant J. 1982; Characterization of two highly oncogenic strains of Marek’s disease virus. Avian Pathology 11:593–605
    [Google Scholar]
  14. Schat K. A., Schinazi R. F., Calnek B. W. 1984; Cell-specific anti-viral activity of l-(2-fluoro-2-deoxy-/l-D-arabinofuranosyl)-5-iodocytosine (FIAC) against Marek’s disease herpesvirus and turkey herpesvirus. Antiviral Research 4:259–270
    [Google Scholar]
  15. Scott S. D. 1990 Identification and sequence analysis of the thymidine kinase and flanking genes in two Marek’s disease herpesviruses Ph.D. thesis University of Cambridge;
    [Google Scholar]
  16. Scott S. D., Ross N. L. J., Binns M. M. 1989; Nucleotide and predicted amino acid sequences of the Marek’s disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. Journal of General Virology 70:3055–3065
    [Google Scholar]
  17. Shih M. F., Arsenakis M., Tiollais P., Roizman B. 1984; Expression of hepatitis B virus S gene by herpes simplex virus type 1 vectors carrying alpha and beta-regulated gene chimeras. Proceedings of the National Academy of Sciences, U.S.A. 81:5867–5870
    [Google Scholar]
  18. Taylor J., Edbauer C., Rey-Senelonge A., Bouquet J. F., Norton E., Goebel S., Desmettre P., Paoletti E. 1990; Newcastle disease virus fusion protein expressed in a fowlpox virus recombinant confers protection in chickens. Journal of Virology 64:1441–1450
    [Google Scholar]
  19. Thompson R. L., Cook M. L., Devi-Rao G. B., Wagner E. K., Stevens J. G. 1986; Functional and molecular analyses of the avirulent wild-type herpes simplex virus type 1 strain KOS. Journal of Virology 58:203–211
    [Google Scholar]
  20. Thomsen D. R., Marotti K. R., Palermo D. P., Post L. E. 1987; Pseudorabies virus as a live virus vector for expression of foreign genes. Gene 57:261–265
    [Google Scholar]
  21. Weise K., Kaerner H. C., Glorioso J., Schroder C. H. 1987; Replacement of glycoprotein B gene sequences in herpes simplex virus type 1 strain ANG by corresponding sequences of the strain KOS causes changes of plaque morphology and neuropathogenicity. Journal of General Virology 68:1909–1919
    [Google Scholar]
  22. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-3-371
Loading
/content/journal/jgv/10.1099/0022-1317-74-3-371
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error