1887

Abstract

An animal model of chronic enteroviral infection was established by using PCR to detect viral genomes in animal tissues and to compare levels of transcription of a variety of cytokines in the brain. Chronic cocksackie-virus B1 infection was found in both brain and skeletal muscle of mice infected as neonates. The viral infection cleared by 240 days post-infection. Elevated levels of tumour necrosis factor α and interleukin-6 (IL-6) would appear to be linked to acute and chronic infection respectively. Levels of IL-6 return to normal upon clearance of the virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-4-741
1993-04-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/4/JV0740040741.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-4-741&mimeType=html&fmt=ahah

References

  1. Archard L. C., Bowles N. E., Behan P. O., Bell E. J., Doyle D. 1988; Postviral fatigue syndrome: persistence of enterovirus RNA in muscle and elevated creatine kinase. Journal of the Royal Society of Medicine 81:326–329
    [Google Scholar]
  2. Bocci V. 1988; Central nervous system toxicity of interferons and other cytokines. Journal of Biological Reagents and Homeostatic Agents 2:107–118
    [Google Scholar]
  3. Chomczynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate phenol chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  4. de la Torre J. C., Borrow P., Oldstone M. BA. 1991; Viral persistence and disease: cytopathology in the absence of cytolysis. British Medical Bulletin 47:838–851
    [Google Scholar]
  5. Frei K., Malipiero U. V., Leist T. P., Zinkernagel R. M., Schwab M. E., Fontana A. 1989; On the central nervous system in viral diseases. European Journal of Immunology 19:689–694
    [Google Scholar]
  6. Gow J. W., Behan W. MH. 1991; Amplification and identification of enteroviral sequences in the postviral fatigue syndrome. British Medical Bulletin 47:872–885
    [Google Scholar]
  7. Haywood A. M. 1986; Patterns of persistent viral infections. New England Journal of Medicine 315:939–948
    [Google Scholar]
  8. Hunter C. A., Gow J. W., Kennedy P. GE., Jennings F. W., Murray M. A. 1991; Immunopathology of experimental African sleeping sickness: detection of cytokine mRNA in the brains of Trypanosoma brucei infected mice. Infection and Immunity 59:4636–4640
    [Google Scholar]
  9. Kandolf R., Ameis D., Kirschner P., Canu A., Hofschneider P. H. 1987; In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridisation: an approach to the diagnosis of viral heart disease. Proceedings of the National Academy of Sciences, U.S.A. 84:6272–6276
    [Google Scholar]
  10. Moskophidis D., Frei K., Lohlerr J., Fontana A., Zinkernagel R. M. 1991; Production of random classes of immunoglobulins in brain tissue during persistent viral infections paralleled by secretion of interleukin-6 (IL-6) but not IL-4, IL-5 and gamma interferon. Journal of Virology 65:1364–1369
    [Google Scholar]
  11. Oldstone M. BA. 1985; An old nemesis in new clothing: viruses playing new tricks by causing cytopathology in the absence of cytolysis. Journal of Infectious Diseases 152:665–667
    [Google Scholar]
  12. Oldstone M. BA., Rodriguez M., Daughaday W. H., Lampert P. W. 1984; Viral perturbation of endocrine function: disorder of cell function leading to disturbed homeostasis and disease. Nature, London 307:278–280
    [Google Scholar]
  13. Patterson P. H. 1992; The emerging neuropoietic cytokine family. Journal of Cellular Biochemistry 16(B):275
    [Google Scholar]
  14. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Strongwater S. L., Dorivini-Zis K., Ball R. D., Schnitzer T. J. 1984; A murine model of polymyositis induced by coxsackievirus B1 (Tucson strain). Arthritis and Rheumatism 27:433–442
    [Google Scholar]
  16. Tam P. E., Schmidt A. M., Ytterberg S. R., Messner R. P. 1991; Viral persistence during the developmental phase of coxsackievirus B1 induced murine polymyositis. Journal of Virology 65:6654–6600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-4-741
Loading
/content/journal/jgv/10.1099/0022-1317-74-4-741
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error