1887

Abstract

In the dorsal root ganglia (DRG) of mice latently infected with the herpes simplex virus type 1 mutant 1814, there are more neurons that contain latency-associated transcripts (LATs) than in DRG of mice infected with a dose of equal infectivity of either a revertant or a wild-type virus. We investigated whether higher levels of LAT neurons resulted in more extensive reactivation either following neurectomy of the sciatic nerve or after explantation into culture. Neurectomy appeared to induce expression of immediate early 1 mRNA (IE1mRNA) in neurons of mice latently infected with each of three viruses. However IE1mRNA was detected in no more than 0.25% of the neurons of DRG from animals 2 to 4 days after neurectomy, irrespective of the percentage of LAT neurons present. Of the 22 neurons shown to express IE1mRNA, none expressed LATs also. However the lack of expression of viral antigen and the absence of a reduced potential for reactivation on explantation suggested that neurectomy had not induced full reactivation involving lytic replication leading to the death of the latently infected neurons. When DRG were explanted into culture, the distribution of the frequency of reactivation was similar to the distribution of DRG that contained LAT neurons. The presence of a high proportion of LAT neurons was not directly associated with earlier detection of reactivation but such experiments cannot be regarded as quantitative. We therefore concluded that neurectomy did not result in a reduced reactivation potential as described by others and that the frequency of expression of IE1mRNA following neurectomy did not correlate with the number of LAT neurons present.

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1993-06-01
2024-03-29
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References

  1. Ace C. I., McKee T. A., Ryan J. M., Cameron J. M., Preston C. M. 1989; Construction and characterization of a herpes simplex virus type 1 mutant unable to transinduce immediate-early gene expression. Journal of Virology 63:2260–2269
    [Google Scholar]
  2. Batchelor A. H., O’Hare P. 1990; Regulation and cell type-specific activity of a promoter located upstream of the latency-associated transcript of herpes simplex virus type 1. Journal of Virology 64:3269–3279
    [Google Scholar]
  3. Bernstein D., Kappes J. C. 1988; Enhanced in vitro reactivation of latent herpes simplex virus from neural and peripheral tissues with hexamethylene bisacetamide. Archives of Virology 99:57–65
    [Google Scholar]
  4. Block T. M., Spivack J. G., Steiner I., Deshmane S., McIntosh M. T., Lirette R. P., Fraser N. W. 1990; A herpes simplex virus type 1 latency-associated transcript mutant reactivates with normal kinetics from latent infection. Journal of Virology 64:3417–3426
    [Google Scholar]
  5. Carton C. A., Kilbourne E. D. 1952; Activation of latent herpes simplex by trigeminal sensory-root section. New England Journal of Medicine 246:172–176
    [Google Scholar]
  6. Deatly A. M., Spivack J. G., Lavi E., Fraser N. W. 1987; RNA from an immediate early region of the type 1 herpes simplex virus genome is present in the trigeminal ganglia of latently infected mice. Proceedings of the National Academy of Sciences, U.S.A. 84:3204–3208
    [Google Scholar]
  7. Ecob-Prince M. S., Preston C. M., Rixon F. J., Hassan K., Kennedy P. G. E. 1993; Neurons containing latency-associated transcripts are numerous and widespread in dorsal root ganglia following footpad inoculation of mice with herpes simplex virus type 1 mutant in1814. Journal of General Virology 74:985–994
    [Google Scholar]
  8. Hill J. M., Sedarati F., Javier R. T., Wagner E. K., Stevens J. G. 1990; Herpes simplex virus latent phase transcription facilitates in vivo reactivation. Virology 174:117–125
    [Google Scholar]
  9. Hill T. J., Blyth W. A., Harbour D. A. 1983; Recurrence of herpes simplex in the mouse requires an intact nerve supply to the skin. Journal of General Virology 64:2763–2765
    [Google Scholar]
  10. Ho D. Y., Mocarski E. S. 1989; Herpes simplex virus latent RNA (LAT) is not required for latent infection in the mouse. Proceedings of the National Academy of Sciences, U.S.A. 86:7596–7600
    [Google Scholar]
  11. Izumi K. M., McKelvey A. M., Devi-Rao G., Wagner E. K., Stevens J. G. 1989; Molecular and biological characterization of a type 1 herpes simplex virus (HSV-1) specifically deleted for expression of the latency-associated transcript (LAT). Microbial Pathogenesis 7:121–134
    [Google Scholar]
  12. Javier R. T., Stevens J. G., Dissette V. B., Wagner E. K. 1988; A herpes simplex virus transcript abundant in latently infected neurons is dispensible for establishment of the latent state. Virology 166:254–257
    [Google Scholar]
  13. Kennedy P. G. E., Al-Saadi S. A., Clements G. B. 1983; Reactivation of latent herpes simplex virus from dissociated identified dorsal root ganglion cells in culture. Journal of General Virology 64:1629–1635
    [Google Scholar]
  14. Ieib D. A., Bogard C. L., Kosz-Vnenchak M., Hicks K. A., Coen D. M., Knipe D. M., Schaffer P. A. 1989; A deletion mutant of the latency-associated transcript of herpes simplex virus 1 reactivates from the latent state with reduced frequency. Journal of Virology 63:2893–2900
    [Google Scholar]
  15. McFarlane M., Daksis J. I., Preston C. M. 1992; Hexamethylene bisacetamide stimulates herpes simplex virus immediate early gene expression in the absence of trans-induction by Vmw65. Journal of General Virology 73:285–292
    [Google Scholar]
  16. McLennan J. L., Darby G. 1980; Herpes simplex virus latency: the cellular location of virus in dorsal root ganglia and the fate of the infected cell following virus activation. Journal of General Virology 51:233–243
    [Google Scholar]
  17. Price R. W., Schmitz J. 1978; Reactivation of latent herpes simplex virus infection of the autonomic nervous system by postganglionic neurectomy. Infection and Immunity 19:523–532
    [Google Scholar]
  18. Rock D., Lokensgard J., Lewis T., Kutish G. 1992; Characterization of dexamethasone-induced reactivation of latent bovine herpesvirus 1. Journal of Virology 66:2484–2490
    [Google Scholar]
  19. Rodahl E., Stevens J. G. 1992; Differential accumulation of herpes simplex virus type 1 latency-associated transcripts in sensory and autonomic ganglia. Virology 189:385–388
    [Google Scholar]
  20. Sawtell N. M., Thompson R. L. 1992a; Rapid in vivo reactivation of herpes simplex virus in latently infected murine ganglionic neurons after transient hyperthermia. Journal of Virology 66:2150–2156
    [Google Scholar]
  21. Sawtell N. M., Thompson R. L. 1992b; Herpes simplex virus type 1 latency-associated transcription unit promotes anatomical site-dependent establishment and reactivation from latency. Journal of Virology 66:2157–2169
    [Google Scholar]
  22. Spivack J. G., Fraser N. W. 1987; Detection of herpes simplex virus type 1 transcripts during latent infection in mice. Journal of Virology 61:3841–3847
    [Google Scholar]
  23. Steiner I., Spivack J. G., Lirette R. P., Brown S. M., MacLean A. R., Subak-Sharpe J. H., Fraser N. W. 1989; Herpes simplex virus type 1 latency-associated transcripts are evidently not essential for latent infection. EM BO Journal 8:505–511
    [Google Scholar]
  24. Steiner I., Spivack J. G., Deshmane S. L., Ace C. I., Preston C. M., Fraser N. W. 1990; A herpes simplex virus type 1 mutant containing a non-transducing Vmw65 protein establishes latent infection in vivo in the absence of viral replication and reactivates efficiently from explanted trigeminal ganglia. Journal of Virology 64:1630–1638
    [Google Scholar]
  25. Stevens J. G., Wagner E. K., Devi-Rao G. B., Cook M. L., Feldman L. T. 1987; RNA complementary to a herpesvirus a-gene mRNA is prominent in latently infected neurons. Science 235:1056–1059
    [Google Scholar]
  26. Tenser R. B., Edris W. A., Hay K. A. 1988; Herpes simplex virus latent infection: reactivation and elimination of latency after neurectomy. Virology 167:302–305
    [Google Scholar]
  27. Trousdale M. D., Steiner I., Spivack J. G., Deshmane S. L., Brown S. M., MacLean A. R., Subak-Sharpe J. H., Fraser N. W. 1991; In vivo and in vitro reactivation impairment of a herpes simplex virus type 1 latency-associated transcription variant in a rabbit eye model. Journal of Virology 65:6989–6993
    [Google Scholar]
  28. Walz M. A., Price R. W., Notkins A. L. 1974; Latent ganglionic infection with herpes simplex virus types 1 and 2: viral reactivation in vivo after neurectomy. Science 184:1185–1187
    [Google Scholar]
  29. Wildy P., Field H. J., Nash A. A. 1982; Classical herpes latency revisited. In Virus Persistence SGM Symposium 33 pp. 133–167 Edited by Mahy B. W. J., Minson A. C., Darby G. K. Cambridge: Cambridge University Press;
    [Google Scholar]
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