1887

Abstract

The temperature-sensitive defect of mutant ts 263 of fowl plague virus (FPV) is located in the acidic polymerase (PA) gene and is due to a single base substitution (C2036T), which leads to an amino acid replacement (Ala to Val) in a highly conserved region of the protein. During passage at 33 °C ts 263 stably carries over a ninth RNA segment, which consists of a truncated PA gene. Although the deletion is in-frame and it is transcribed into mRNA, no corresponding protein is detected . After reversion to wild-type this extra RNA segment is immediately lost. At the nonpermissive temperature of 40 °C no significant viral products of ts 263 are synthesized. Under semi-permissive conditions there is a relative, but very significant over-production of the M1 protein, which is not accompanied by a corresponding elevated M1 mRNA synthesis. These results are in agreement with the idea that the PA protein is involved in the regulation of viral protein synthesis at the level of expression of mRNA. Preinfection of chicken embryo cells with ts 263 at a semi-permissive temperature interferes with the replication of FPV wild-type indicating that premature availability of M1 might be detrimental for influenza virus replication.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-9-1789
1993-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/9/JV0740091789.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-9-1789&mimeType=html&fmt=ahah

References

  1. Aminoff D. 1961; Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.. Biochemical Journal 81:384–392
    [Google Scholar]
  2. Becht H, Malole B. 1975; Comparative evaluation of different fixation procedures and different coupling reagents for the demon-straction of influenza virus-specific antibodies by the direct hemagglutination test.. Medical Microbiology and Immunology 162:43–53
    [Google Scholar]
  3. Bosch FX, Orlich M, Klenk HD, Rott R. 1979; The structure of the hemagglutinin, a determinant for the pathogenicity of influenza viruses.. Virology 9:197–207
    [Google Scholar]
  4. Braam J, Ulmanen I, Krug RM. 1983; Molecular model of a eukaryotic transcription complex: functions and movements of influenza P proteins during capped RNA-primed transcription.. Cell 34:609–618
    [Google Scholar]
  5. Brownlee GG, Cartwright FM. 1977; Rapid gel sequencing of RNA by primed synthesis with reverse transcriptase.. Journal of Molecular Biology 114:93
    [Google Scholar]
  6. Chomczynski P, Sacchi N. 1987; Single step method of RNA isolation by acidic guanidinium thiocyanate-phenol-chloroform extraction.. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. Enami M, Fukuda R, Ishihama A. 1985; Transcription and replication of eight RNA segments of influenza virus.. Virology 142:68–77
    [Google Scholar]
  8. Fischer AAH, Muller K, Scholtissek C. 1990; Specific inhibition of the synthesis of influenza late proteins and stimulation of early, M2, and NS2 protein synthesis by 3-deazaadenosine.. Virology 177:523–531
    [Google Scholar]
  9. Goldman D, Merril CR. 1982; Silver staining of DNA in polyacrylamide gels: linearity and effect of fragment size.. Electrophoresis 3:24–26
    [Google Scholar]
  10. Hankins RW, Nagata K, Bucher DJ, Popple SS, Ishihama A. 1989; Monoclonal antibody analysis of influenza virus matrix protein epitopes involved in transcription inhibition.. Vims Genes 3:111–126
    [Google Scholar]
  11. Hatada E, Hasegawa M, Mukaigawa J, Shimizu K, Fukuda R. 1989; Control of influenza virus gene expression: quantitative analysis of each viral RNA species in infected cells.. Journal of Biochemistry 105:537–564
    [Google Scholar]
  12. Hatada E, Hasegawa M, Mukaigawa J, Shimizu K, Hatanaka M, Fukuda R. 1990; Analysis of influenza A virus temperature-sensitive mutants with mutations in RNA segment 8.. Journal of General Virology 71:1283–1292
    [Google Scholar]
  13. Hay AJ, Lomniczi B, Bellamy AR, Skehel JJ. 1977; Transcription of the influenza virus genome.. Virology 83:337–355
    [Google Scholar]
  14. Jacobson AB, Good L, Zucker M. 1984; Some simple computational methods to improve the folding of large RNAs.. Nucleic Acids Research 12:45–52
    [Google Scholar]
  15. Klenk HD, Rott R, Becht H. 1972; Virology 4. Nucleic Acids Research579–591
    [Google Scholar]
  16. Koennecke I, Boschek CB, Scholtissek C. 1981; Isolation and properties of a temperature-sensitive mutant (ts 412) of an influenza A virus recombinant with a ts lesion in the gene coding for the nonstructural protein.. Virology 110:16–25
    [Google Scholar]
  17. Ludwig S, Schultz U, Mandler J, Scholtissek C. 1991; Phylogenetic relationship of the nonstructural (NS) gene of influenza A virus.. Virology 110:16–25
    [Google Scholar]
  18. Mahy BWJ. 1983; Mutants of influenza virus.. In Genetics of Influenza Viruses pp. 192–254Edited by Palese P., Kingsbury D. W. Wien & New York: Springer-Verlag;
    [Google Scholar]
  19. Maniatis T, Fritsch E. F, Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Martin K, Helenius A. 1991; Transport of incoming influenza virus nucleocapsids into the nucleus.. Journal of Virology 65:232–244
    [Google Scholar]
  21. Okazaki K, Kawaoka Y, Webster RG. 1989; Evolutionary pathways of the PA genes of influenza A viruses.. Virology 172:601–608
    [Google Scholar]
  22. Robertson JS, Robertson ME, St C, Room IJ. 1984; Nucleotide sequence of RNA segment 3 of the avian influenza A/FPV/Rostock/34 and its comparison with the corresponding segment of human strains A/PR8/34 and A/NT/60/68.. Virus Research 1:73–79
    [Google Scholar]
  23. Sanger F, Nicklen S, Coulson AR. 1977; DNA sequencing with chain-terminating inhibitors.. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  24. Scholtissek C, Bowles AL. 1975; Isolation and characterization of temperature-sensitive mutants of fowl plague virus.. Virology 67:576–587
    [Google Scholar]
  25. Scholtissek C, Spring SB. 1982; Extragenic suppression of temperature-sensitive mutations in RNA segment 8 by replacement of different RNA segments with those of other influenza A virus prototype strains.. Virology 118:28–34
    [Google Scholar]
  26. Scholtissek C, Harms E, Rhode W, Orlich M, Rott R. 1976; Correlation between RNA fragments of fowl plague virus and their corresponding gene functions.. Virology 74:322–344
    [Google Scholar]
  27. Schultz U, Fitch WM, Ludwig S, Mandler J, Scholtissek C. 1991; Evolution of pig influenza viruses.. Virology 183:61–73
    [Google Scholar]
  28. Shapiro GI, Gurney TJ, Krug RM. 1987; Influenza virus gene expression: control mechanisms at early and late times of infection and nuclear transport of virus specific RNAs.. Journal of Virology 61:764–773
    [Google Scholar]
  29. Smith GL, Hay AJ. 1982; Replication of the influenza virus genome.. Virology 118:96–108
    [Google Scholar]
  30. Tabor R, Richardson CC. 1987; DNA sequence analysis with a modified bacteriophage T7 polymerase.. Proceedings of the National Academy of Sciences, U.S.A. 84:4767–4771
    [Google Scholar]
  31. Wahl GM, Stern M, Stark GR. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.. Proceedings of the National Academy of Sciences, U.S.A. 76:3683–3687
    [Google Scholar]
  32. Wolstenholme AJ, Barrett T, Nichol ST, Mahy BWJ. 1980; Influenza virus specific RNA and protein synthesis in cells infected with temperature-sensitive mutants defective in the genome segment encoding the nonstructural proteins.. Journal of Virology 35:1–7
    [Google Scholar]
  33. Ye Z, Baylor NW, Wagner RR. 1989; Transcription-inhibition and RNA-binding domains of influenza A virus matrix protein mapped with anti-idiotypic antibodies and synthetic peptides.. Journal of Virology 63:3586–3594
    [Google Scholar]
  34. Zvonarjev AY, Ghendon YZ. 1980; Influence of the membrane (M) protein on influenza A virus virion transcriptase activity in vitro and its susceptibility to rimantadine.. Journal of Virology 33:583–586
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-74-9-1789
Loading
/content/journal/jgv/10.1099/0022-1317-74-9-1789
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error