f The DNA sequence of the equine herpesvirus 4 gene encoding glycoprotein gp17/18, the homologue of herpes simplex virus glycoprotein gD
- Authors: A. A. Cullinane, J. Neilan†, L. Wilson‡, A. J. Davison, G. Allen
- First Published Online: 01 September 1993, Journal of General Virology 74: 1959-1964, doi: 10.1099/0022-1317-74-9-1959
- Subject: Animal
- Issue Published:
The nucleotide sequence of the gene to the left of the gI gene of equine herpesvirus 4 (EHV-4) was determined. The gene encodes a peptide of 402 amino acids with an unprocessed M r of 45323. The predicted polypeptide has several features of a glycoprotein including a hydrophobic signal sequence, a membrane spanning domain and four potential N-linked glycosylation sites within the proposed external domain. The predicted amino acid sequence of EHV-4 gD shows 83% identity with that of equine herpesvirus 1 gD. Conservation of the tertiary structure is suggested by the alignment of six cysteine residues with those of the gD of six other alphaherpesviruses. Screening a λgt11/EHV-4 expression library with monoclonal antibodies against several of the most abundant EHV-4 glycoproteins unequivocally identified the protein encoded by the EHV-4 gD gene as gp17/18.
Present address: Plum Island Disease Center, P.O. Box 848, Greenport, New York 11944, U.S.A.
Present address: AFRC Institute for Animal Health, Compton, Newbury, Berkshire RG16 0NN, U.K.
© Society for General Microbiology 1993 | Published by the Microbiology Society
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