@article{mbs:/content/journal/jgv/10.1099/0022-1317-75-1-65, author = "Lanciotti, Robert S. and Lewis, Joyce Grant and Gubler, Duane J. and Trent, Dennis W.", title = "Molecular evolution and epidemiology of dengue-3 viruses", journal= "Journal of General Virology", year = "1994", volume = "75", number = "1", pages = "65-75", doi = "https://doi.org/10.1099/0022-1317-75-1-65", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-1-65", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The nucleic acid sequences of the pre-membrane/ membrane and envelope protein genes of 23 geographically and temporally distinct dengue (DEN)-3 viruses were determined. This was accomplished by reverse transcriptase-PCR amplification of the structural genes followed by automated DNA sequence analysis. Comparison of nucleic acid sequences revealed that similarity among the viruses was greater than 90 %. The similarity among deduced amino acids was between 95 % and 100 %, and in many cases identical amino acid substitutions occurred among viruses from similar geographical regions. Alignment of nucleic acid sequences followed by parsimony analysis allowed the generation of phylo-genetic trees, demonstrating that geographically independent evolution of DEN-3 viruses had occurred. The DEN-3 viruses were separated into four genetically distinct subtypes. Subtype I consists of viruses from Indonesia, Malaysia, the Philippines and the South Pacific islands; subtype II consists of viruses from Thailand; subtype III consists of viruses from Sri Lanka, India, Africa and Samoa; subtype IV consists of viruses from Puerto Rico and the 1965 Tahiti virus. Phylogenetic analysis has also contributed to our understanding of the molecular epidemiology and worldwide distribution of DEN-3 viruses.", }