1887

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Volume 75, Issue 10

infection of human hepatoma cells (HepG2) with hepatitis B virus (HBV): spontaneous selection of a stable HBV surface antigen-producing HepG2 cell line containing integrated HBV DNA sequences Free

Abstract

The degree of susceptibility of human hepatoma (HepG2) cells to direct hepatitis B virus (HBV) infection remains unknown. We previously observed a low level of Dane particle production and viral DNA replication after infection of HepG2 cells with serum-derived HBV. However, this culture system appeared to be affected by variations as human hepatocyte cultures. In the present study, HBV infection of HepG2 cells led to a significant increase in the secretion of three envelope antigens (HBsAg, preS2Ag and preSl Ag) at 4 days post-infection, and Northern blot analysis revealed the presence of both preSl (2·6 kb) and preS2/S (2·2 kb) transcripts. Expression of preSlAg and the corresponding viral RNA became undetectable on 21 days post-infection whereas the 2·2 kb RNA species persisted and was associated with secretion of subviral HBs particles expressing preS2-epitopes and banding between 30 and 35% sucrose. At 35 days post-infection (fifth passage), a sudden high level production of HBsAg and preSlAg was observed, followed by a massive cell death (90 %). A stable HBsAg-producing HepG2 cell line, designated HepG2-BV3, grew out of the surviving cells. HepG2- BV3 cells could integrate HBV DNA sequences and produce the three HBV surface antigens. Treatment with dexamethasone increased the HBsAg and preSlAg secretion. Such a HBsAg-producing HepG2 cell line obtained by HBV infection seems to mimick events that occur in the naturally occurring persistent chronic infection, and therefore may be an efficient model for studying the contribution of viral integration in the dysregulation of HBV and liver- specific genes expression.

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© The Journal of General Virology 1994
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1994-10-01
2024-04-19
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In vitro infection of human hepatoma cells (HepG2) with hepatitis B virus (HBV): spontaneous selection of a stable HBV surface antigen-producing HepG2 cell line containing integrated HBV DNA sequences
J. Gen. Virol. 75, 2681 (1994); https://doi.org/10.1099/0022-1317-75-10-2681
/content/journal/jgv/10.1099/0022-1317-75-10-2681
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